Human periostin/osteoblast specific factor 2, POSTN ELISA Kit | MyBioSource MBS705827 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human periostin/osteoblast specific factor 2, POSTN ELISA Kit

catalog :

MBS705827

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS705827

products type :

ELISA Kit

products full name :

Human periostin/osteoblast specific factor 2, POSTN ELISA Kit

products short name :

periostin/osteoblast specific factor 2 (POSTN)

products name syn :

Human periostin/osteoblast specific factor 2 (POSTN) ELISA kit; MGC119510; MGC119511; OSF-2; OSF2; PDLPOSTN; PN; RP11-412K4.1; periostin; osteoblast specific factor 2 (fasciclin I-like) ; periodontal ligament-specific periostin; periostin isoform thy2; periostin iso; periostin/osteoblast specific factor 2 (POSTN)

other names :

periostin isoform 2; Periostin; periostin; periodontal ligament-specific periostin; osteoblast specific factor 2 (fasciclin I-like); periostin, osteoblast specific factor; Osteoblast-specific factor 2; OSF-2

products gene name :

POSTN

other gene names :

POSTN; POSTN; PN; OSF2; OSF-2; PDLPOSTN; periostin; OSF2; PN; OSF-2

uniprot entry name :

POSTN_HUMAN

reactivity :

Human

sequence length :

779

specificity :

This assay has high sensitivity and excellent specificity for detection of human POSTN. No significant cross-reactivity or interference between human POSTN and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 6.25 ng/ml -400 ng/ml. Sensitivity: The minimum detectable dose of human POSTN is typically less than 1.56 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for POSTN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any POSTN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POSTN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POSTN bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

209862911

ncbi acc num :

NP_001129406.1

ncbi gb acc num :

NM_001135934.1

uniprot acc num :

Q15063

ncbi mol weight :

93,314 Da

ncbi pathways :

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

ncbi summary :

This gene encodes a secreted extracellular matrix protein that functions in tissue development and regeneration, including wound healing, and ventricular remodeling following myocardial infarction. The encoded protein binds to integrins to support adhesion and migration of epithelial cells. This protein plays a role in cancer stem cell maintenance and metastasis. Mice lacking this gene exhibit cardiac valve disease, and skeletal and dental defects. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

uniprot summary :

Function: Enhances incorporation of BMP1 in the fibronectin matrix of connective tissues, and subsequent proteolytic activation of lysyl oxidase LOX Induces cell attachment and spreading and plays a role in cell adhesion. May play a role in extracellular matrix mineralization.2 PublicationsManual assertion based on experiment in:Ref.2

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage