product summary
Loading...
company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Alanine Aminotransferase, ALT/GPT ELISA Kit
catalog :
MBS705799
quantity :
48-Strip-Wells
price :
565 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS705799
products type :
ELISA Kit
products full name :
Mouse Alanine Aminotransferase, ALT/GPT ELISA Kit
products short name :
Alanine Aminotransferase (ALT/GPT)
products name syn :
Mouse Alanine Aminotransferase (ALT/GPT) ELISA Kit; AAT1; ALT1; GPT1; glutamic pyruvate transaminase; glutamic-alanine transaminase 1; Alanine Aminotransferase (ALT/GPT)
other names :
alanine aminotransferase 1; Alanine aminotransferase 1; alanine aminotransferase 1; ALT1; GPT 1; glutamic--alanine transaminase 1; glutamic--pyruvic transaminase 1; glutamate pyruvate transaminase 1; glutamic pyruvic transaminase 1, soluble; glutamic pyruvic transaminase, soluble; Glutamate pyruvate transaminase 1; GPT 1; Glutamic--alanine transaminase 1; Glutamic--pyruvic transaminase 1
products gene name :
ALT/GPT
other gene names :
Gpt; Gpt; ALT; Gpt1; Gpt-1; 1300007J06Rik; 2310022B03Rik; Gpt1; ALT1; GPT 1
uniprot entry name :
ALAT1_MOUSE
reactivity :
Mouse
sequence length :
496
specificity :
This assay has high sensitivity and excellent specificity for detection of Mouse ALT/GPT. No significant cross-reactivity or interference between Mouse ALT/GPT and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, Cell lysates. Detection Range: 0.156 mU/ml-10 mU/ml. Sensitivity: 0.039 mU/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ALT. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for ALT and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain ALT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of ALT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi gi num :
33413404
ncbi acc num :
NP_877957.1
ncbi gb acc num :
NM_182805.2
uniprot acc num :
Q8QZR5
ncbi mol weight :
55,143 Da
ncbi pathways :
2-Oxocarboxylic Acid Metabolism Pathway (715633); 2-Oxocarboxylic Acid Metabolism Pathway (717400); Alanine And Aspartate Metabolism Pathway (198303); Alanine, Aspartate And Glutamate Metabolism Pathway (101155); Alanine, Aspartate And Glutamate Metabolism Pathway (100063); Amino Acid Synthesis And Interconversion (transamination) Pathway (970564); Biosynthesis Of Amino Acids Pathway (790952); Biosynthesis Of Amino Acids Pathway (795174); Carbon Metabolism Pathway (815838); Carbon Metabolism Pathway (817567)
uniprot summary :
Function: Catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Participates in cellular nitrogen metabolism and also in liver gluconeogenesis starting with precursors transported from skeletal muscles . Catalytic activity: L-alanine + 2-oxoglutarate = pyruvate + L-glutamate. Cofactor: Pyridoxal phosphate
size1 :
48-Strip-Wells
price1 :
565 USD
size2 :
96-Strip-Wells
price2 :
810
size3 :
5x96-Strip-Wells
price3 :
2750
size4 :
10x96-Strip-Wells
price4 :
5150
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!