ELISA/assay

Human Xaa-Pro Dipeptidase/Prolidase, PEPD ELISA Kit

MBS705782

48-Strip-Wells

565 USD

ELISA Kit

Human Xaa-Pro Dipeptidase/Prolidase, PEPD ELISA Kit

peptidase D

Human Xaa-Pro Dipeptidase/Prolidase (PEPD) ELISA Kit; MGC10905; PROLIDASE; aminoacyl-L-proline hydrolase; imidodipeptidase; prolidase; proline dipeptidase; peptidase D

xaa-Pro dipeptidase isoform 1; Xaa-Pro dipeptidase; xaa-Pro dipeptidase; imidodipeptidase; X-Pro dipeptidase; proline dipeptidase; aminoacyl-L-proline hydrolase; peptidase D; Imidodipeptidase; Peptidase D; Proline dipeptidase; Prolidase

PEPD

PEPD; PEPD; PROLIDASE; PRD; X-Pro dipeptidase; Prolidase

PEPD_HUMAN

Human

493

This assay has high sensitivity and excellent specificity for detection of Human PEPD. No significant cross-reactivity or interference between Human PEPD and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 93.75 mU/ml-6000 mU/ml. Sensitivity: 23.4 mU/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Developmental Protein. Protein Biological Process 3: Collagen degradation

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PEPD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PEPD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PEPD is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PEPD bound in the initial step. The color development is stopped and the intensity of the color is measured.

149589008

NP_000276.2

NM_000285.3

P12955

54,548 Da

Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)

This gene encodes a member of the peptidase family. The protein forms a homodimer that hydrolyzes dipeptides or tripeptides with C-terminal proline or hydroxyproline residues. The enzyme serves an important role in the recycling of proline, and may be rate limiting for the production of collagen. Mutations in this gene result in prolidase deficiency, which is characterized by the excretion of large amount of di- and tri-peptides containing proline. Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Oct 2009]

Function: Splits dipeptides with a prolyl or hydroxyprolyl residue in the C-terminal position. Plays an important role in collagen metabolism because the high level of iminoacids in collagen. Catalytic activity: Hydrolysis of Xaa- -Pro dipeptides; also acts on aminoacyl-hydroxyproline analogs. No action on Pro- -Pro. Cofactor: Binds 2 manganese ions per subunit.1 PublicationManual assertion based on experiment in:Ref.10

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150