Fish Acetylcholinesterase, AChE ELISA Kit | MyBioSource MBS705766 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Fish Acetylcholinesterase, AChE ELISA Kit

catalog :

MBS705766

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS705766

products type :

ELISA Kit

products full name :

Fish Acetylcholinesterase, AChE ELISA Kit

products short name :

Acetylcholinesterase (AChE)

products name syn :

Fish Acetylcholinesterase (AChE) ELISA Kit; ARACHE; N-ACHE; YT; OTTHUMP00000211347; OTTHUMP00000211349; OTTHUMP00000211356; acetylcholinesterase; apoptosis-related acetylcholinesterase; Acetylcholinesterase (AChE)

other names :

acetylcholinesterase isoform E4-E6; Acetylcholinesterase; acetylcholinesterase; Yt blood group; apoptosis-related acetylcholinesterase; acetylcholinesterase (Yt blood group)

products gene name :

AChE

other gene names :

ACHE; ACHE; YT; ACEE; ARACHE; N-ACHE; AChE

uniprot entry name :

ACES_HUMAN

reactivity :

Fish

sequence length :

614

specificity :

This assay has high sensitivity and excellent specificity for detection of Fish AChE. No significant cross-reactivity or interference between Fish AChE and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 2.5 ng/ml-40 ng/ml. Sensitivity: 1.25 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Neurobiology. Protein Biological Process 3: Neurotransmitter degradation

products description :

Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A antibody specific to AChE has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated AChE and incubated. A competitive inhibition reaction is launched between AChE (Standards or samples) and Biotin-conjugated AChE with the pre-coated antibody specific for AChE. The more amount of AChE in samples, the less antibody bound by Biotin-conjugated AChE. Then Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of AChE in the sample. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4557239

ncbi acc num :

NP_000656.1

ncbi gb acc num :

NM_000665.3

uniprot acc num :

P22303

ncbi mol weight :

67,796 Da

ncbi pathways :

ATF-2 Transcription Factor Network Pathway (138006); Acetylcholine Synthesis Pathway (198820); Biogenic Amine Synthesis Pathway (198793); Cholinergic Synapse Pathway (217716); Glycerophospholipid Biosynthesis Pathway (645313); Glycerophospholipid Metabolism Pathway (82989); Glycerophospholipid Metabolism Pathway (364); Integrated Pancreatic Cancer Pathway (711360); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976)

ncbi summary :

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft. Role in neuronal apoptosis.4 PublicationsManual assertion based on experiment in:Ref.11. Catalytic activity: Acetylcholine + H2O = choline + acetate.

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage