catalog number :
MBS705761
products type :
ELISA Kit
products full name :
Mouse plasma kallikrein, KLKB1 ELISA Kit
products short name :
plasma kallikrein (KLKB1)
products name syn :
Mouse plasma kallikrein (KLKB1) ELISA Kit; KLK3; PPK; Fletcher factor; kininogenin; plasma kallikrein B1; plasma kallikrein heavy chain; plasma kallikrein light chain; plasma kallikrein (KLKB1)
other names :
plasma kallikrein; Plasma kallikrein; plasma kallikrein; kininogenin; fletcher factor; kallikrein 3, plasma; plasma prekallikrein; antigen, prostate specific; kallikrein B, plasma 1; Fletcher factor; Kininogenin; Plasma prekallikreinCleaved into the following 2 chains:Plasma kallikrein heavy chain; Plasma kallikrein light chain
products gene name :
KLKB1
other gene names :
Klkb1; Klkb1; APS; PSA; Kal3; Klk3; Kal-3; Klk3; Pk
uniprot entry name :
KLKB1_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse KLKB1. No significant cross-reactivity or interference between mouse KLKB1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 ng/ml-200 ng/ml. Sensitivity: Typically less than 0.78 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KLKB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KLKB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KLKB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KLKB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_032481.2
ncbi gb acc num :
NM_008455.2
ncbi mol weight :
71,383 Da
ncbi pathways :
Activation Of Matrix Metalloproteinases Pathway (1001029); Complement And Coagulation Cascades Pathway (198335); Complement And Coagulation Cascades Pathway (83270); Complement And Coagulation Cascades Pathway (484); Degradation Of The Extracellular Matrix Pathway (1001028); Extracellular Matrix Organization Pathway (1001015); Formation Of Fibrin Clot (Clotting Cascade) Pathway (1001318); Hemostasis Pathway (1001295); Intrinsic Pathway (1001320)
uniprot summary :
Function: The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. Catalytic activity: Cleaves selectively Arg- -Xaa and Lys- -Xaa bonds, including Lys- -Arg and Arg- -Ser bonds in (human) kininogen to release bradykinin. Enzyme regulation: Inhibited by SERPINA5
size4 :
10x96-Strip-Wells