Human Semaphorin 3A, Sema 3A ELISA Kit | MyBioSource MBS705755 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Semaphorin 3A, Sema 3A ELISA Kit

catalog :

MBS705755

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS705755

products type :

ELISA Kit

products full name :

Human Semaphorin 3A, Sema 3A ELISA Kit

products short name :

sema domain, immunoglobulin domain (Ig) , short basic domain, secreted, (semaphorin) 3A

products name syn :

Human Semaphorin 3A (Sema 3A) ELISA Kit; Hsema-I; Hsema-III; MGC133243; SEMA1; SEMAD; SEMAIII; SEMAL; SemD; coll-1; OTTHUMP00000208884; collapsin 1; semaphorin 3A; semaphorin D; semaphorin III; semaphorin L; semaphorin-like; sema domain; immunoglobulin domain (Ig); short basic domain; secreted; (semaphorin) 3A

other names :

semaphorin-3A; Semaphorin-3A; semaphorin-3A; collapsin 1; semaphorin D; semaphorin L; semaphorin III; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3A; Semaphorin III; Sema III

products gene name :

SEMA3A

other gene names :

SEMA3A; SEMA3A; HH16; SemD; COLL1; SEMA1; SEMAD; SEMAL; coll-1; Hsema-I; SEMAIII; Hsema-III; SEMAD; Sema III

uniprot entry name :

SEM3A_HUMAN

reactivity :

Human

sequence length :

771

specificity :

This assay has high sensitivity and excellent specificity for detection of human Sema 3A. No significant cross-reactivity or interference between human Sema 3A and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: 0.039 ng/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Sema 3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Sema 3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Sema 3A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Sema 3A bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

5174673

ncbi acc num :

NP_006071.1

ncbi gb acc num :

NM_006080.2

uniprot acc num :

Q14563

ncbi mol weight :

88,889 Da

ncbi pathways :

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); CRMPs In Sema3A Signaling Pathway (119525); Developmental Biology Pathway (477129); SEMA3A-Plexin Repulsion Signaling By Inhibiting Integrin Adhesion Pathway (119523); Sema3A PAK Dependent Axon Repulsion Pathway (119524); Semaphorin Interactions Pathway (119519)

ncbi summary :

This gene is a member of the semaphorin family and encodes a protein with an Ig-like C2-type (immunoglobulin-like) domain, a PSI domain and a Sema domain. This secreted protein can function as either a chemorepulsive agent, inhibiting axonal outgrowth, or as a chemoattractive agent, stimulating the growth of apical dendrites. In both cases, the protein is vital for normal neuronal pattern development. Increased expression of this protein is associated with schizophrenia and is seen in a variety of human tumor cell lines. Also, aberrant release of this protein is associated with the progression of Alzheimer's disease. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Involved in the development of the olfactory system and in neuronal control of puberty. Induces the collapse and paralysis of neuronal growth cones. Could serve as a ligand that guides specific growth cones by a motility-inhibiting mechanism. Binds to the complex neuropilin-1/plexin-1.1 PublicationManual assertion based on experiment in:Ref.3

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage