Human Neurofilament protein L, NF-L ELISA Kit | MyBioSource MBS705750 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Neurofilament protein L, NF-L ELISA Kit

catalog :

MBS705750

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS705750

products type :

ELISA Kit

products full name :

Human Neurofilament protein L, NF-L ELISA Kit

products short name :

neurofilament, light polypeptide

products name syn :

Human Neurofilament protein L (NF-L) ELISA kit; CMT1F; CMT2E; FLJ53642; NF-L; NF68; NFL; light molecular weight neurofilament protein; neurofilament protein; light chain; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament; lig; neurofilament; light polypeptide

other names :

neurofilament light polypeptide; Neurofilament light polypeptide; neurofilament light polypeptide; neurofilament subunit NF-L; neurofilament triplet L protein; neurofilament protein, light chain; neurofilament, light polypeptide 68kDa; light molecular weight neurofilament protein; protein phosphatase 1, regulatory subunit 110; neurofilament, light polypeptide; 68 kDa neurofilament protein; Neurofilament triplet L protein

products gene name :

NEFL

other gene names :

NEFL; NEFL; NFL; NF-L; NF68; CMT1F; CMT2E; PPP1R110; NF68; NFL; NF-L

uniprot entry name :

NFL_HUMAN

reactivity :

Human

sequence length :

543

specificity :

This assay has high sensitivity and excellent specificity for detection of human NF-L. No significant cross-reactivity or interference between human NF-L and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: Typically less than 7.8 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NF-L has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NF-L present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NF-L is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NF-L bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

105990539

ncbi acc num :

NP_006149.2

ncbi gb acc num :

NM_006158.4

uniprot acc num :

P07196

ncbi mol weight :

61,517 Da

ncbi pathways :

Activation Of NMDA Receptor Upon Glutamate Binding And Postsynaptic Events Pathway (161033); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975); Amyotrophic Lateral Sclerosis (ALS) Pathway (83099); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); CREB Phosphorylation Through The Activation Of CaMKII Pathway (161040); CREB Phosphorylation Through The Activation Of Ras Pathway (161036); Neuronal System Pathway (106513); Neurotransmitter Receptor Binding And Downstream Transmission In The Postsynaptic Cell Pathway (106534); Post NMDA Receptor Activation Events Pathway (161035); Ras Activation Uopn Ca2+ Infux Through NMDA Receptor Pathway (161037)

ncbi summary :

Neurofilaments are type IV intermediate filament heteropolymers composed of light, medium, and heavy chains. Neurofilaments comprise the axoskeleton and they functionally maintain the neuronal caliber. They may also play a role in intracellular transport to axons and dendrites. This gene encodes the light chain neurofilament protein. Mutations in this gene cause Charcot-Marie-Tooth disease types 1F (CMT1F) and 2E (CMT2E), disorders of the peripheral nervous system that are characterized by distinct neuropathies. A pseudogene has been identified on chromosome Y. [provided by RefSeq, Oct 2008]

uniprot summary :

Function: Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage