catalog number :
MBS705737
products type :
ELISA Kit
products full name :
Rat Insulin-Like 3, INSL3 ELISA Kit
products short name :
insulin-like 3 (Leydig cell)
products name syn :
Rat Insulin-Like 3 (INSL3) ELISA Kit; MGC119818; MGC119819; RLF; RLNL; insulin-like 3; leydig insulin -like hormone; leydig insulin -like peptide; relaxin-like factor b; insulin-like 3 (Leydig cell)
other names :
insulin-like 3; Insulin-like 3; insulin-like 3; ley-I-L; relaxin-like factor; Leydig insulin-like peptide relaxin-like factor; insulin-like 3; Leydig insulin-like peptide; Ley-I-L; Relaxin-like factorCleaved into the following 2 chains:Insulin-like 3 B chain; Insulin-like 3 A chain
products gene name :
INSL3
other gene names :
Insl3; Insl3; Rlf; Rlf; Ley-I-L
uniprot entry name :
INSL3_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat INSL3. No significant cross-reactivity or interference between rat INSL3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for INSL3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any INSL3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for INSL3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of INSL3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_446132.1
ncbi gb acc num :
NM_053680.1
ncbi mol weight :
14,110 Da
ncbi pathways :
Class A/1 (Rhodopsin-like Receptors) Pathway (1010164); G Alpha (s) Signalling Events Pathway (1010194); GPCR Downstream Signaling Pathway (1010193); GPCR Ligand Binding Pathway (1010163); Peptide Ligand-binding Receptors Pathway (1010165); Relaxin Receptors Pathway (1010171); Signal Transduction Pathway (1010015); Signaling By GPCR Pathway (1010162)
ncbi summary :
ligand for LGR8; functions in testis descent [RGD, Feb 2006]
uniprot summary :
Function: Seems to play a role in testicular function. May be a trophic hormone with a role in testicular descent in fetal life. Is a ligand for LGR8 receptor
size4 :
10x96-Strip-Wells