catalog number :
MBS705730
products type :
ELISA Kit
products full name :
Human Semaphorin 4C, Sema 4C ELISA Kit
products short name :
sema domain, immunoglobulin domain (Ig) , transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C
products name syn :
Human Semaphorin 4C (Sema 4C) ELISA Kit; FLJ20369; KIAA1739; M-SEMA-F; MGC126382; MGC126383; SEMACL1; SEMAF; SEMAI; M-Sema F; semaphorin 4C; sema domain; immunoglobulin domain (Ig); transmembrane domain (TM) and short cytoplasmic domain; (semaphorin) 4C
other names :
semaphorin-4C; Semaphorin-4C; semaphorin-4C; M-Sema F; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4C
products gene name :
SEMA4C
other gene names :
SEMA4C; SEMA4C; SEMAF; SEMAI; SEMACL1; M-SEMA-F; KIAA1739; SEMAI; UNQ5855/PRO34487
uniprot entry name :
SEM4C_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human SEMA4C. No significant cross-reactivity or interference between Human SEMA4C and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Developmental Protein. Protein Biological Process 3: Differentiation
products description :
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sema 4C. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Sema 4C and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Sema 4C, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Sema 4C in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi acc num :
NP_060259.4
ncbi gb acc num :
NM_017789.4
ncbi mol weight :
92,623 Da
ncbi pathways :
Axon Guidance Pathway (83065); Axon Guidance Pathway (476)
uniprot summary :
Function: Cell surface receptor for PLXNB2 that plays an important role in cell-cell signaling. PLXNB2 binding promotes downstream activation of RHOA and phosphorylation of ERBB2 at 'Tyr-1248'. Required for normal brain development, axon guidance and cell migration Probable signaling receptor which may play a role in myogenic differentiation through activation of the stress-activated MAPK cascade.1 PublicationManual assertion based on experiment in:Ref.4
size4 :
10x96-Strip-Wells