catalog number :
MBS705689
products type :
ELISA Kit
products full name :
Human Mucin3, MUC3 ELISA Kit
products short name :
mucin 17, cell surface associated
products name syn :
Human Mucin3 (MUC3) ELISA Kit; MUC3; membrane mucin MUC17; mucin 17; secreted mucin MUC17; small intestinal mucin MUC3; mucin 17; cell surface associated
other names :
mucin-17; Mucin-17; mucin-17; MUC-3; MUC-17; membrane mucin MUC17; secreted mucin MUC17; small intestinal mucin-3; small intestinal mucin MUC3; mucin 17, cell surface associated; Small intestinal mucin-3; MUC-3
products gene name :
MUC17
other gene names :
MUC17; MUC17; MUC3; MUC3; MUC-17; MUC-3
uniprot entry name :
MUC17_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human MUC3. No significant cross-reactivity or interference between human MUC3 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20 ng/ml. Sensitivity: 0.078 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MUC3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUC3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUC3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUC3 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001035194.1
ncbi gb acc num :
NM_001040105.1
ncbi mol weight :
451,741 Da
ncbi pathways :
Metabolism Of Proteins Pathway (106230); O-linked Glycosylation Pathway (980459); O-linked Glycosylation Of Mucins Pathway (530747); Post-translational Protein Modification Pathway (161012); Termination Of O-glycan Biosynthesis Pathway (530748)
ncbi summary :
Membrane mucins, such as MUC17, function in epithelial cells to provide cytoprotection, maintain luminal structure, provide signal transduction, and confer antiadhesive properties upon cancer cells that lose their apical/basal polarization.[supplied by OMIM, Apr 2004]
uniprot summary :
Function: Probably plays a role in maintaining homeostasis on mucosal surfaces.1 PublicationManual assertion based on experiment in:Ref.6
size4 :
10x96-Strip-Wells