ELISA/assay

Mouse insulin-like growth factors 1 receptor, IGF-1R ELISA Kit

MBS705678

96 Strip Wells

760 USD

ELISA Kit

Mouse insulin-like growth factors 1 receptor, IGF-1R ELISA Kit

insulin-like growth factor 1 receptor

Mouse insulin-like growth factors 1 receptor (IGF-1R) ELISA Kit; CD221; IGFIR; IGFR; JTK13; MGC142170; MGC142172; MGC18216; soluble IGF1R variant 1; soluble IGF1R variant 2; insulin-like growth factor 1 receptor

insulin-like growth factor 1 receptor; Insulin-like growth factor 1 receptor; insulin-like growth factor 1 receptor; line 186; IGF-I receptor; insulin-like growth factor I receptor; Insulin-like growth factor I receptor; IGF-I receptor; CD_antigen: CD221Cleaved into the following 2 chains:Insulin-like growth factor 1 receptor alpha chain; Insulin-like growth factor 1 receptor beta chain

IGF1R

Igf1r; Igf1r; hyft; CD221; IGF-1R; D930020L01; A330103N21Rik; IGF-I receptor

IGF1R_MOUSE

Mouse

1369

This assay has high sensitivity and excellent specificity for detection of Mouse IGF1R. No significant cross-reactivity or interference between Mouse IGF1R and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm

Sample Volume: 50-100ul

112983656

NP_034643.2

NM_010513.2

Q60751

155,788 Da

AMPK Signaling Pathway 989651!!AMPK Signaling Pathway 992181!!Adherens Junction Pathway 83267!!Adherens Junction Pathway 481!!Apoptosis Pathway 198339!!Endochondral Ossification Pathway 198336!!Endocytosis Pathway 102286!!Endocytosis Pathway 102181!!Focal Adhesion Pathway 83264!!Focal Adhesion Pathway 478

Function: Receptor tyrosine kinase which mediates actions of insulin-like growth factor 1 (IGF1). Binds IGF1 with high affinity and IGF2 and insulin (INS) with a lower affinity. The activated IGF1R is involved in cell growth and survival control. IGF1R is crucial for tumor transformation and survival of malignant cell. Ligand binding activates the receptor kinase, leading to receptor autophosphorylation, and tyrosines phosphorylation of multiple substrates, that function as signaling adapter proteins including, the insulin-receptor substrates (IRS1/2), Shc and 14-3-3 proteins. Phosphorylation of IRSs proteins lead to the activation of two main signaling pathways: the PI3K-AKT/PKB pathway and the Ras-MAPK pathway. The result of activating the MAPK pathway is increased cellular proliferation, whereas activating the PI3K pathway inhibits apoptosis and stimulates protein synthesis. Phosphorylated IRS1 can activate the 85 kDa regulatory subunit of PI3K (PIK3R1), leading to activation of several downstream substrates, including protein AKT/PKB. AKT phosphorylation, in turn, enhances protein synthesis through mTOR activation and triggers the antiapoptotic effects of IGFIR through phosphorylation and inactivation of BAD. In parallel to PI3K-driven signaling, recruitment of Grb2/SOS by phosphorylated IRS1 or Shc leads to recruitment of Ras and activation of the ras-MAPK pathway. In addition to these two main signaling pathways IGF1R signals also through the Janus kinase/signal transducer and activator of transcription pathway (JAK/STAT). Phosphorylation of JAK proteins can lead to phosphorylation/activation of signal transducers and activators of transcription (STAT) proteins. In particular activation of STAT3, may be essential for the transforming activity of IGF1R. The JAK/STAT pathway activates gene transcription and may be responsible for the transforming activity. JNK kinases can also be activated by the IGF1R. IGF1 exerts inhibiting activities on JNK activation via phosphorylation and inhibition of MAP3K5/ASK1, which is able to directly associate with the IGF1R When present in a hybrid receptor with INSR, binds IGF1 . Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Enzyme regulation: Activated by autophosphorylation at Tyr-1163, Tyr-1167 and Tyr-1168 on the kinase activation loop; phosphorylation at all three tyrosine residues is required for optimal kinase activity. Inhibited by MSC1609119A-1, BMS-754807, PQIP, benzimidazole pyridinone, isoquinolinedione, bis-azaindole, 3-cyanoquinoline, 2,4-bis-arylamino-1,3-pyrimidine, pyrrolopyrimidine, pyrrole-5-carboxaldehyde, picropodophyllin (PPP), tyrphostin derivatives. While most inhibitors bind to the ATP binding pocket, MSC1609119A-1 functions as allosteric inhibitor and binds close to the DFG motif and the activation loop 1 PublicationManual assertion based on experiment in:Ref.5

96 Strip Wells

760 USD