product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Sheep Interleukin 4, IL-4 ELISA Kit
catalog :
MBS705670
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
citations: 1
Reference
Tan J, Chan S, Lo C, Deane J, McDonald C, Bernard C, et al. Amnion cell-mediated immune modulation following bleomycin challenge: controlling the regulatory T cell response. Stem Cell Res Ther. 2015;6:8 pubmed publisher
product information
catalog number :
MBS705670
products type :
ELISA Kit
products full name :
Sheep Interleukin 4, IL-4 ELISA Kit
products short name :
interleukin 4
products name syn :
Sheep Interleukin 4 (IL-4) ELISA Kit; BCGF-1; BCGF1; BSF1; IL-4; MGC79402; B cell growth factor 1; B_cell stimulatory factor 1; lymphocyte stimulatory factor 1; interleukin 4
other names :
interleukin-4; Interleukin-4; interleukin-4; BSF-1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell stimulatory factor 1; BSF-1; Lymphocyte stimulatory factor 1
products gene name :
IL4
other gene names :
IL4; IL4; IL-4; IL-4; IL-4; BSF-1
uniprot entry name :
IL4_SHEEP
reactivity :
Sheep
sequence length :
135
specificity :
This assay has high sensitivity and excellent specificity for detection of sheep IL-4. No significant cross-reactivity or interference between sheep IL-4 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Detection Range: 6.25 pg/ml -400 pg/ml. Sensitivity: The minimum detectable dose of sheep IL-4 is typically less than 1.56 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Assay Type: Sandwich
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IL-4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IL-4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
57527820
ncbi acc num :
NP_001009313.2
ncbi gb acc num :
NM_001009313.2
uniprot acc num :
P30368
ncbi mol weight :
15,136 Da
ncbi pathways :
Allograft Rejection Pathway (84530); Allograft Rejection Pathway (535); Asthma Pathway (84527); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (84528); Autoimmune Thyroid Disease Pathway (533); Cytokine-cytokine Receptor Interaction Pathway (84463); Cytokine-cytokine Receptor Interaction Pathway (460); Fc Epsilon RI Signaling Pathway (84494); Fc Epsilon RI Signaling Pathway (493)
uniprot summary :
Function: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes.
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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