catalog number :
MBS705653
products type :
ELISA Kit
products full name :
Rat Alpha 2 Heremans Schmid Glycoprotein (AHSG) ELISA Kit
products short name :
alpha-2-HS-glycoprotein
other names :
alpha-2-HS-glycoprotein
products gene name :
AHSG
products gene name syn :
A2HS; AHS; FETUA; HSGA; fetuin-A
specificity :
This assay recognizes rat AHSG. No significant cross-reactivity or interference was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Sensitivity: The minimum detectable dose of rat AHSG is typically less than 125 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero.
products description :
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with AHSG. Standards or samples are then added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) -conjugated antibody preparation specific for AHSG, mix well and incubated. The more the amount of AHSG in samples, the less HRP- conjugated antibody preparation specific for AHSG bound by pre-coated AHSG. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. And the color develops in opposite to the amount of AHSG in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
AAA51683.1
uniprot summary :
FETUA: Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Shows affinity for calcium and barium ions. Alpha-2-HS glycoprotein derives from this precursor, when the connecting peptide is cleaved off. The two chains A and B are held together by a single disulfide bond. Synthesized in liver and selectively concentrated in bone matrix. Secreted in plasma. It is also found in dentin in much higher quantities than other plasma proteins. Belongs to the fetuin family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 3q27. Cellular Component: extracellular space; extracellular region. Molecular Function: kinase inhibitor activity; cysteine protease inhibitor activity. Biological Process: pinocytosis; negative regulation of bone mineralization; ossification; positive regulation of phagocytosis; regulation of inflammatory response; acute-phase response; negative regulation of insulin receptor signaling pathway; negative regulation of phosphorylation; skeletal development; regulation of bone mineralization
size4 :
10x96-Strip-Wells