catalog number :
MBS705643
products type :
ELISA Kit
products full name :
Canine heart fatty acid binding protein, h-FABP ELISA Kit
products short name :
heart fatty acid binding protein (h-FABP)
products name syn :
Canine heart fatty acid binding protein (h-FABP) ELISA Kit; heart fatty acid binding protein (h-FABP)
other names :
heart-type fatty acid binding protein; Fatty acid-binding protein, heart; Fatty acid-binding protein 3; Heart-type fatty acid-binding protein; H-FABP
products gene name :
h-FABP
other gene names :
FABP3; H-FABP
uniprot entry name :
FABPH_MYOLU
specificity :
This assay has high sensitivity and excellent specificity for detection of canine h-FABP. No significant cross-reactivity or interference between canine h-FABP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml -20ng/ml. Sensitivity: The minimum detectable dose of canine h-FABP is typically less than 0.078 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for h-FABP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any h-FABP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for h-FABP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of h-FABP bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
AAO49500.1
ncbi mol weight :
14,814 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: FABP are thought to play a role in the intracellular transport of long-chain fatty acids and their acyl-CoA esters. FABPs are important elements related to the hibernating state in mammals.
size4 :
10x96-Strip-Wells