ELISA/assay

Mouse Nuclear factor erythroid 2-related factor 2, Nrf2 ELISA Kit

MBS705641

96 Strip Wells

850 USD

ELISA Kit

Mouse Nuclear factor erythroid 2-related factor 2, Nrf2 ELISA Kit

nuclear factor (erythroid-derived 2) -like 2

Mouse Nuclear factor erythroid 2-related factor 2 (Nrf2) ELISA Kit; NRF2; NF-E2-related factor 2; nuclear factor erythroid 2-like 2; nuclear factor (erythroid-derived 2) -like 2

nuclear factor erythroid 2-related factor 2; Nuclear factor erythroid 2-related factor 2; nuclear factor erythroid 2-related factor 2; NFE2-related factor 2; NF-E2-related factor 2; nuclear factor, erythroid derived 2, like 2; Nuclear factor, erythroid derived 2, like 2

NFE2L2

Nfe2l2; Nfe2l2; Nrf2; AI194320; Nrf-2; Nrf2; NF-E2-related factor 2; NFE2-related factor 2

NF2L2_MOUSE

Mouse

597

This assay has high sensitivity and excellent specificity for detection of Mouse NFE2L2. No significant cross-reactivity or interference between Mouse NFE2L2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma and cell culture supernates. Detection Range: 0.312 ng/ml-20 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.078 ng/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Transcription/Transcription regulation. Protein Biological Process 3: Transcription

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear factor erythroid 2-related factor 2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Nuclear factor erythroid 2-related factor 2 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain Nuclear factor erythroid 2-related factor 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Nuclear factor erythroid 2-related factor 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

6754832

NP_035032.1

NM_010902.3

Q60795

66,901 Da

Keap1-Nrf2 Pathway 198378!!Protein Processing In Endoplasmic Reticulum Pathway 167331!!Protein Processing In Endoplasmic Reticulum Pathway 167190!!Selenium Metabolism/Selenoproteins Pathway 198289

Function: Transcription activator that binds to antioxidant response (ARE) elements in the promoter regions of target genes. Important for the coordinated up-regulation of genes in response to oxidative stress. May be involved in the transcriptional activation of genes of the beta-globin cluster by mediating enhancer activity of hypersensitive site 2 of the beta-globin locus control region.1 PublicationManual assertion based on experiment in:Ref.6

96 Strip Wells

850 USD