Human Fibroblast Growth Factor 8, FGF8 ELISA Kit | MyBioSource MBS705620 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Fibroblast Growth Factor 8, FGF8 ELISA Kit

catalog :

MBS705620

quantity :

48-Strip-Wells

price :

435 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS705620

products type :

ELISA Kit

products full name :

Human Fibroblast Growth Factor 8, FGF8 ELISA Kit

products short name :

fibroblast growth factor 8 (androgen-induced)

products name syn :

Human Fibroblast Growth Factor 8 (FGF8) ELISA Kit; AIGF; HBGF-8; KAL6; MGC149376; androgen-induced growth factor; fibroblast growth factor 8; fibroblast growth factor 8 (androgen-induced)

other names :

fibroblast growth factor 8 isoform B; Fibroblast growth factor 8; fibroblast growth factor 8; androgen-induced growth factor; heparin-binding growth factor 8; fibroblast growth factor 8 (androgen-induced); Androgen-induced growth factor; AIGF; Heparin-binding growth factor 8; HBGF-8

products gene name :

FGF8

other gene names :

FGF8; FGF8; HH6; AIGF; KAL6; FGF-8; HBGF-8; AIGF; FGF-8; AIGF; HBGF-8

uniprot entry name :

FGF8_HUMAN

reactivity :

Human

sequence length :

215

specificity :

This assay has high sensitivity and excellent specificity for detection of Human FGF8. No significant cross-reactivity or interference between Human FGF8 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Developmental Protein. Protein Biological Process 3: Differentiation

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FGF8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGF8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

5174439

ncbi acc num :

NP_006110.1

ncbi gb acc num :

NM_006119.4

uniprot acc num :

P55075

ncbi mol weight :

26,525 Da

ncbi pathways :

Activated Point Mutants Of FGFR2 Pathway (645281); Adaptive Immune System Pathway (366160); Constitutive PI3K/AKT Signaling In Cancer Pathway (685535); DAP12 Interactions Pathway (685549); DAP12 Signaling Pathway (685550); Disease Pathway (530764); Downstream Signal Transduction Pathway (106385); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (576250); Downstream Signaling Of Activated FGFR Pathway (160957); FGFR Ligand Binding And Activation Pathway (106344)

ncbi summary :

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is known to be a factor that supports androgen and anchorage independent growth of mammary tumor cells. Overexpression of this gene has been shown to increase tumor growth and angiogensis. The adult expression of this gene is restricted to testes and ovaries. Temporal and spatial pattern of this gene expression suggests its function as an embryonic epithelial factor. Studies of the mouse and chick homologs revealed roles in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination. The alternative splicing of this gene results in four transcript variants. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: Plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. Required for normal brain, eye, ear and limb development during embryogenesis. Required for normal development of the gonadotropin-releasing hormone (GnRH) neuronal system.3 PublicationsManual assertion based on experiment in:Ref.9

size1 :

48-Strip-Wells

price1 :

435 USD

size2 :

96-Strip-Wells

price2 :

615

size3 :

5x96-Strip-Wells

price3 :

2340

size4 :

10x96-Strip-Wells

price4 :

4370

more info or order :

MyBioSource product webpage