ELISA/assay

Rat Fibroblast Growth Factor 10, FGF10 ELISA Kit

MBS705610

48-Strip-Wells

510 USD

ELISA Kit

Rat Fibroblast Growth Factor 10, FGF10 ELISA Kit

fibroblast growth factor 10

Rat Fibroblast Growth Factor 10 (FGF10) ELISA Kit; keratinocyte growth factor 2; produced by fibroblasts of urinary bladder lamina propria; fibroblast growth factor 10

fibroblast growth factor 10; Fibroblast growth factor 10; fibroblast growth factor 10; fibroblast growth factor 10

FGF10

Fgf10; Fgf10; FGF-10; FGF-10

FGF10_RAT

Rat

215

This assay has high sensitivity and excellent specificity for detection of rat FGF10. No significant cross-reactivity or interference between rat FGF10 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml -1000 pg/ml. Sensitivity: The minimum detectable dose of rat FGF10 is typically less than 3.9 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FGF10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGF10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

6978837

NP_037083.1

NM_012951.1

P70492

24,029 Da

Activated Point Mutants Of FGFR2 Pathway (1010497); Adaptive Immune System Pathway (1010925); Constitutive PI3K/AKT Signaling In Cancer Pathway (1010535); DAP12 Interactions Pathway (1011029); DAP12 Signaling Pathway (1011030); Developmental Biology Pathway (1009870); Disease Pathway (1010484); Downstream Signal Transduction Pathway (1010117); Downstream Signaling Events Of B Cell Receptor (BCR) Pathway (1010939); Downstream Signaling Of Activated FGFR Pathway (1010048)

binds the KGFR receptor, a splice variant of FGFR-2; may contribute to tissue regeneration and angiogenesis after acute pancreatitis [RGD, Feb 2006]

Function: Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. May play a role in wound healing.

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800