Human Hydroxymethylglutaryl CoA Reductase, HMG-CoAR ELISA Kit | MyBioSource MBS705606 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Hydroxymethylglutaryl CoA Reductase, HMG-CoAR ELISA Kit

catalog :

MBS705606

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS705606

products type :

ELISA Kit

products full name :

Human Hydroxymethylglutaryl CoA Reductase, HMG-CoAR ELISA Kit

products short name :

3-hydroxy-3-methylglutaryl-Coenzyme A reductase

products name syn :

Human Hydroxymethylglutaryl CoA Reductase (HMG-CoAR) ELISA kit; LDLCQ3; 3-hydroxy-3-methylglutaryl CoA reductase (NADPH) ; hydroxymethylglutaryl-CoA reductase; 3-hydroxy-3-methylglutaryl-Coenzyme A reductase

other names :

3-hydroxy-3-methylglutaryl-Coenzyme A reductase isoform 1; 3-hydroxy-3-methylglutaryl-coenzyme A reductase; 3-hydroxy-3-methylglutaryl-Coenzyme A reductase; HMG-CoA reductase; hydroxymethylglutaryl-CoA reductase; 3-hydroxy-3-methylglutaryl CoA reductase (NADPH); 3-hydroxy-3-methylglutaryl-CoA reductase

products gene name :

HMGCR

other gene names :

HMGCR; HMGCR; LDLCQ3; HMG-CoA reductase

uniprot entry name :

HMDH_HUMAN

reactivity :

Human

sequence length :

888

specificity :

This assay has high sensitivity and excellent specificity for detection of Human HMGCR. No significant cross-reactivity or interference between Human HMGCR and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.78 ng/ml- 50 ng/ml. Sensitivity: 0.195 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Biosynthesis/Metabolism. Protein Biological Process 2: Lipogenesis and lipometabolism. Protein Biological Process 3: Cholesterol biosynthesis

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HMG-CoAR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HMG-CoAR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HMG-CoAR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HMG-CoAR bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4557643

ncbi acc num :

NP_000850.1

ncbi gb acc num :

NM_000859.2

uniprot acc num :

P04035

ncbi mol weight :

97,476 Da

ncbi pathways :

AMPK Signaling Pathway (198868); AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Activation Of Gene Expression By SREBF (SREBP) Pathway (685552); Bile Secretion Pathway (193146); Bile Secretion Pathway (193095); C5 Isoprenoid Biosynthesis, Mevalonate Pathway (413387); C5 Isoprenoid Biosynthesis, Mevalonate Pathway (468288); Cholesterol Biosynthesis Pathway (198809); Cholesterol Biosynthesis Pathway (106142)

ncbi summary :

HMG-CoA reductase is the rate-limiting enzyme for cholesterol synthesis and is regulated via a negative feedback mechanism mediated by sterols and non-sterol metabolites derived from mevalonate, the product of the reaction catalyzed by reductase. Normally in mammalian cells this enzyme is suppressed by cholesterol derived from the internalization and degradation of low density lipoprotein (LDL) via the LDL receptor. Competitive inhibitors of the reductase induce the expression of LDL receptors in the liver, which in turn increases the catabolism of plasma LDL and lowers the plasma concentration of cholesterol, an important determinant of atherosclerosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2008]

uniprot summary :

Function: Transmembrane glycoprotein that is the rate-limiting enzyme in cholesterol biosynthesis as well as in the biosynthesis of nonsterol isoprenoids that are essential for normal cell function including ubiquinone and geranylgeranyl proteins. Catalytic activity: (R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH. Enzyme regulation: Regulated by a negative feedback mechanism through sterols and non-sterol metabolites derived from mevalonate. Inhibited by statins, a class of hypolipidemic agents used as pharmaceuticals to lower cholesterol levels in individuals at risk from cardiovascular disease due to hypercholesterolemia. Inhibition of HMGCR in the liver stimulates the LDL-receptors, which results in an increased clearance of LDL from the bloodstream and a decrease in blood cholesterol levels. The first results can be seen after one week of statin use and the effect is maximal after four to six weeks.1 PublicationManual assertion based on experiment in:Ref.7

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage