ELISA/assay

Human melanoma-associated antigen 3, MAGE-3 ELISA Kit

MBS705585

48-Strip-Wells

510 USD

ELISA Kit

Human melanoma-associated antigen 3, MAGE-3 ELISA Kit

melanoma antigen family A, 3

Human melanoma-associated antigen 3 (MAGE-3) ELISA Kit; CT1.3; HIP8; HYPD; MAGE3; MAGEA6; MGC14613; MAGE-3 antigen; antigen MZ2-D; cancer/testis antigen family 1; member 3; melanoma-associated antigen 3; melanoma antigen family A; 3

melanoma-associated antigen 3; Melanoma-associated antigen 3; melanoma-associated antigen 3; antigen MZ2-D; MAGE-3 antigen; cancer/testis antigen 1.3; cancer/testis antigen family 1, member 3; melanoma antigen family A, 3; Antigen MZ2-D; Cancer/testis antigen 1.3; CT1.3; MAGE-3 antigen

MAGEA3

MAGEA3; MAGEA3; HIP8; HYPD; CT1.3; MAGE3; MAGEA6; MAGE3; CT1.3

MAGA3_HUMAN

Human

314

This assay has high sensitivity and excellent specificity for detection of human MAGE-3. No significant cross-reactivity or interference between human MAGE-3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, urine, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: 0.156 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MAGE-3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MAGE-3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MAGE-3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MAGE-3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4885467

NP_005353.1

NM_005362.3

P43357

34,747 Da

This gene is a member of the MAGEA gene family. The members of this family encode proteins with 50 to 80% sequence identity to each other. The promoters and first exons of the MAGEA genes show considerable variability, suggesting that the existence of this gene family enables the same function to be expressed under different transcriptional controls. The MAGEA genes are clustered at chromosomal location Xq28. They have been implicated in some hereditary disorders, such as dyskeratosis congenita. [provided by RefSeq, Jul 2008]

Function: Proposed to enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. May enhance ubiquitin ligase activity of TRIM28 and stimulate p53/TP53 ubiquitination by TRIM28. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. May play a role in embryonal development and tumor transformation or aspects of tumor progression. In vitro promotes cell viability in melanoma cell lines. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes.1 PublicationManual assertion based on experiment in:Ref.9

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800