Rat Soluble Lectin-like Oxidized Low Density Lipoprotein Receptor-1, sLOX-1 ELISA Kit | MyBioSource MBS705578 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Soluble Lectin-like Oxidized Low Density Lipoprotein Receptor-1, sLOX-1 ELISA Kit

catalog :

MBS705578

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS705578

products type :

ELISA Kit

products full name :

Rat Soluble Lectin-like Oxidized Low Density Lipoprotein Receptor-1, sLOX-1 ELISA Kit

products short name :

oxidized low density lipoprotein (lectin-like) receptor 1

products name syn :

Rat Soluble Lectin-like Oxidized Low Density Lipoprotein Receptor-1 (sLOX-1) ELISA Kit; CLEC8A; LOX1; SCARE1; lectin-type oxidized LDL receptor 1; oxidised low density lipoprotein (lectin-like) receptor 1; scavenger receptor class E; member 1; oxidized low density lipoprotein (lectin-like) receptor 1

other names :

oxidized low-density lipoprotein receptor 1; Oxidized low-density lipoprotein receptor 1; oxidized low-density lipoprotein receptor 1; ox-LDL receptor 1; lectin-like oxLDL receptor 1; lectin-like oxidized LDL receptor 1; lectin-type oxidized LDL receptor 1; Lectin-like oxidized LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1; Lectin-type oxidized LDL receptor 1Cleaved into the following chain:Oxidized low-density lipoprotein receptor 1, soluble form

products gene name :

OLR1

other gene names :

OLR1; OLR1; LOX1; LOX-1; LOX1; Ox-LDL receptor 1; LOX-1; Lectin-like oxLDL receptor 1

uniprot entry name :

OLR1_RABIT

reactivity :

Rat

sequence length :

278

specificity :

This assay has high sensitivity and excellent specificity for detection of rat sLOX-1. No significant cross-reactivity or interference between rat sLOX-1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of rat sLOX-1 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sLOX-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sLOX-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sLOX-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sLOX-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

130493907

ncbi acc num :

NP_001076102.1

ncbi gb acc num :

NM_001082633.1

uniprot acc num :

Q9XTA8

ncbi mol weight :

31,159 Da

ncbi pathways :

Cell Surface Interactions At The Vascular Wall Pathway (106062); Hemostasis Pathway (106028); PPAR Signaling Pathway (83042); PPAR Signaling Pathway (450); Phagosome Pathway (153910); Phagosome Pathway (153859)

uniprot summary :

Function: Receptor that mediates the recognition, internalization and degradation of oxidatively modified low density lipoprotein (oxLDL) by vascular endothelial cells. OxLDL is a marker of atherosclerosis that induces vascular endothelial cell activation and dysfunction, resulting in pro-inflammatory responses, pro-oxidative conditions and apoptosis. Its association with oxLDL induces the activation of NF-kappa-B through an increased production of intracellular reactive oxygen and a variety of pro-atherogenic cellular responses including a reduction of nitric oxide (NO) release, monocyte adhesion and apoptosis. In addition to binding oxLDL, it acts as a receptor for the HSP70 protein involved in antigen cross-presentation to naive T-cells in dendritic cells, thereby participating in cell-mediated antigen cross-presentation. Also involved in inflammatory process, by acting as a leukocyte-adhesion molecule at the vascular interface in endotoxin-induced inflammation. Also acts as a receptor for advanced glycation end (AGE) products, activated platelets, monocytes, apoptotic cells and both Gram-negative and Gram-positive bacteria

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage