catalog number :
MBS705561
products type :
ELISA Kit
products full name :
Bovine IgE ELISA Kit
products short name :
IgE
products name syn :
Bovine IgE ELISA Kit; IgE
specificity :
This assay has high sensitivity and excellent specificity for detection of bovine IgE. No significant cross-reactivity or interference between bovine IgE and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 62.5 ng/ml -4000 ng/ml. Sensitivity: 15.6 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for IgE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IgE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IgE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IgE bound in the initial step. The color development is stopped and the intensity of the color is measured.
size4 :
10x96-Strip-Wells