ELISA/assay

Bovine Heat Shock Protein 72, HSP-72 ELISA Kit

MBS705541

96 Strip Wells

850 USD

ELISA Kit

Bovine Heat Shock Protein 72, HSP-72 ELISA Kit

heat shock 70kDa protein 2

Bovine Heat Shock Protein 72 (HSP-72) ELISA Kit; HSP70-2; HSP70-3; OTTHUMP00000180664; heat shock 70kD protein 2; heat shock 70kDa protein 2

heat shock-related 70 kDa protein 2; Heat shock-related 70 kDa protein 2; heat shock-related 70 kDa protein 2; HSP70.3; heat shock 70 kD protein 3; heat shock 70kDa protein 3; heat shock 70 kDa protein 3; heat shock 70kDa protein 1A; heat shock 70kDa protein 2<; Heat shock 70 kDa protein 3; HSP70.3

HSPA2

HSPA2; HSPA2; HSPA3; HSP70-3; HSPA3; HSP70.3

HSP72_BOVIN

Bovine

631

This assay has high sensitivity and excellent specificity for detection of Bovine HSPA2. No significant cross-reactivity or interference between Bovine HSPA2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 0.312 ng/ml-20 ng/ml. Detection Wavelength: 450 nm. Sensitivity: 0.078 ng/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Neurobiology. Protein Biological Process 3: Stress response

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to trypsinogen 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for trypsinogen 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain trypsinogen 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of trypsinogen 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

41386699

NP_776769.1

NM_174344.1

P34933

69,740 Da

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Function: In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage.

96 Strip Wells

850 USD