ELISA/assay

Human Soluble Cluster of Differentiation 80, sCD80 ELISA Kit

MBS705535

48-Strip-Wells

565 USD

ELISA Kit

Human Soluble Cluster of Differentiation 80, sCD80 ELISA Kit

CD80 molecule

Human Soluble Cluster of Differentiation 80 (sCD80) ELISA Kit; CD28LG; CD28LG1; LAB7; B-lymphocyte activation antigen B7; CD80 antigen; CD80 antigen (CD28 antigen ligand 1; B7-1 antigen) ; costimulatory factor CD80; costimulatory molecule variant IgV-CD80; CD80 molecule

T-lymphocyte activation antigen CD80; T-lymphocyte activation antigen CD80; T-lymphocyte activation antigen CD80; activation B7-1 antigen; costimulatory factor CD80; CTLA-4 counter-receptor B7.1; B-lymphocyte activation antigen B7; costimulatory molecule variant IgV-CD80; CD80 antigen (CD28 antigen ligand 1, B7-1 antigen); CD80 molecule; Activation B7-1 antigen; BB1; CTLA-4 counter-receptor B7.1; B7; CD_antigen: CD80

CD80

CD80; CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1; CD28LG; CD28LG1; LAB7; B7

CD80_HUMAN

Human

288

This assay has high sensitivity and excellent specificity for detection of human sCD80. No significant cross-reactivity or interference between human sCD80 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, cell culture supernates. Assay Type: Sandwich. Detection Range: 125 pg/ml -8000 pg/ml. Sensitivity: The minimum detectable dose of human sCD80 is typically less than 31.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sCD80 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sCD80 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sCD80 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sCD80 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4885123

NP_005182.1

NM_005191.3

P33681

33,048 Da

Adaptive Immune System Pathway (366160); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); CD28 Co-stimulation Pathway (119553); CD28 Dependent PI3K/Akt Signaling Pathway (119554); CD28 Dependent Vav1 Pathway (119555); CTLA4 Inhibitory Signaling Pathway (119556)

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]

Function: Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.1 PublicationManual assertion based on experiment in:Ref.8

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150