ELISA/assay

Human Paraoxonase 3, PON3 ELISA Kit

MBS705533

48-Strip-Wells

510 USD

ELISA Kit

Human Paraoxonase 3, PON3 ELISA Kit

paraoxonase 3

Human Paraoxonase 3 (PON3) ELISA Kit; paraoxanase-3; serum paraoxonase/lactonase 3; paraoxonase 3

serum paraoxonase/lactonase 3; Serum paraoxonase/lactonase 3; serum paraoxonase/lactonase 3; paraoxanase-3; arylesterase 3; paraoxonase 3

PON3

PON3; PON3

PON3_HUMAN

Human

354

This assay has high sensitivity and excellent specificity for detection of human PON3. No significant cross-reactivity or interference between human PON3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: 0.039 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PON3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PON3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PON3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PON3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

29788996

NP_000931.1

NM_000940.2

Q15166

39,607 Da

Metabolic Pathways (132956); Phase I, Non P450 Pathway (198854)

This gene is a member of the paraoxonase family and lies in a cluster on chromosome 7 with the other two family members. The encoded protein is secreted into the bloodstream and associates with high-density lipoprotein (HDL). The protein also rapidly hydrolyzes lactones and can inhibit the oxidation of low-density lipoprotein (LDL), a function that is believed to slow the initiation and progression of atherosclerosis. Alternatively spliced variants which encode different protein isoforms have been described; however, only one has been fully characterized. [provided by RefSeq, Jul 2008]

Function: Has low activity towards the organophosphate paraxon and aromatic carboxylic acid esters. Rapidly hydrolyzes lactones such as statin prodrugs (e.g. lovastatin). Hydrolyzes aromatic lactones and 5- or 6-member ring lactones with aliphatic substituents but not simple lactones or those with polar substituents.1 PublicationManual assertion based on experiment in:Ref.9. Catalytic activity: A phenyl acetate + H2O = a phenol + acetate.1 PublicationManual assertion based on experiment in:Ref.9. Cofactor: Binds 2 calcium ions per subunit

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800