catalog number :
MBS705531
products type :
ELISA Kit
products full name :
Mouse Eosinophil Peroxidase, EPO ELISA Kit
products short name :
eosinophil peroxidase
products name syn :
Mouse Eosinophil Peroxidase (EPO) ELISA Kit; EPO; EPP; EPX-PEN; ; eosinophil peroxidase
other names :
eosinophil peroxidase; Eosinophil peroxidase; eosinophil peroxidase; eosinophil peroxidase
other gene names :
Epx; Epx; EPO; Eper; EPO
uniprot entry name :
PERE_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse EPO. No significant cross-reactivity or interference between mouse EPO and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: 0.039 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EPO has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EPO present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EPO is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EPO bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_031972.2
ncbi gb acc num :
NM_007946.2
ncbi mol weight :
81,380 Da
ncbi pathways :
Asthma Pathway (83313); Asthma Pathway (532); Phosphate Acquisition II Pathway (542835)
uniprot summary :
Function: Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils 1 PublicationManual assertion based on experiment in:Ref.4. Catalytic activity: 2 phenolic donor + H2O2 = 2 phenoxyl radical of the donor + 2 H2O. Cofactor: Binds 1 calcium ion per heterodimer
size4 :
10x96-Strip-Wells