catalog number : 
MBS705517
products type : 
ELISA Kit
products full name : 
Mouse Hydroxymethylglutaryl CoA Reductase, HMG-CoAR ELISA Kit
products short name : 
Hydroxymethylglutaryl CoA Reductase (HMG-CoAR)
products name syn : 
Mouse Hydroxymethylglutaryl CoA Reductase (HMG-CoAR) ELISA kit; Hydroxymethylglutaryl CoA Reductase (HMG-CoAR)
other names : 
HMG CoA-reductase, partial; HMG CoA-reductase; HMG CoA-reductase; HMG CoA-reductase
products gene name : 
HMG-CoAR
other gene names : 
hmg-coa; HMG-CoA
uniprot entry name : 
A5HL74_ORYLA
specificity : 
This assay has high sensitivity and excellent specificity for detection of Mouse HMG-CoAR. No significant cross-reactivity or interference between Mouse HMG-CoAR and analogues was observed.
storage stability : 
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 : 
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess.  Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess.  Sample Type: serum, plasma, cell culture supernates, cell lysates.  Detection Range: 0.156 ng/ml-10 ng/ml.  Detection Wavelength: 450 nm.  Sensitivity: 0.039 ng/ml
other info2 : 
Sample Volume: 50-100ul
products description : 
Principle of the Assay:  This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HMG-CoAR has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HMG-CoAR present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HMG-CoAR is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HMG-CoAR bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num : 
ABQ09275.1
ncbi mol weight : 
42,979 Da
ncbi pathways : 
C5 Isoprenoid Biosynthesis, Mevalonate Pathway  750618!!C5 Isoprenoid Biosynthesis, Mevalonate Pathway  468288!!Metabolic Pathways  750373!!Terpenoid Backbone Biosynthesis Pathway  750305!!Terpenoid Backbone Biosynthesis Pathway  408