ELISA/assay

Pig Mucin-2, MUC2 ELISA Kit

MBS705512

48-Strip-Wells

515 USD

ELISA Kit

Pig Mucin-2, MUC2 ELISA Kit

Mucin-2, MUC2

Pig Mucin-2; MUC2 ELISA Kit; Mucin-2; MUC2

mucin-2; Mucin-2; mucin-2; intestinal mucin-2; mucin-like protein; mucin 2, intestinal/tracheal; mucin 2, oligomeric mucus/gel-forming; Intestinal mucin-2

MUC2

MUC2; MUC2; MLP; SMUC; MUC-2; SMUC; MUC-2

MUC2_HUMAN

Pig

5179

This assay has high sensitivity and excellent specificity for detection of pig mucin-2. No significant cross-reactivity or interference between pig mucin-2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 ng/ml-500 ng/ml. Sensitivity: The minimum detectable dose of pig mucin-2 is typically less than 1.95 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for mucin-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any mucin-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for mucin-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of mucin-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

116284392

NP_002448.2

NM_002457.2

Q02817

540,300 Da

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Metabolism Of Proteins Pathway (106230); O-linked Glycosylation Pathway (980459); O-linked Glycosylation Of Mucins Pathway (530747); Post-translational Protein Modification Pathway (161012); Termination Of O-glycan Biosynthesis Pathway (530748); Vibrio Cholerae Infection Pathway (83101); Vibrio Cholerae Infection Pathway (513)

This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Jul 2008]

Function: Coats the epithelia of the intestines, airways, and other mucus membrane-containing organs. Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. Major constituent of both the inner and outer mucus layers of the colon and may play a role in excluding bacteria from the inner mucus layer.1 PublicationManual assertion based on experiment in:Ref.11

48-Strip-Wells

515 USD

96-Strip-Wells

735

5x96-Strip-Wells

2600

10x96-Strip-Wells

4865