catalog number :
MBS705506
products type :
ELISA Kit
products full name :
Human Cytochrome P450 ELISA Kit
products short name :
Cytochrome P450
products name syn :
Human Cytochrome P450 ELISA kit; Cytochrome P450
other names :
cytochrome P450; cytochrome P450
products gene name :
CYP450
other gene names :
SCO0801; SCF43.12
specificity :
This assay has high sensitivity and excellent specificity for detection of human Cytochrome P450. No significant cross-reactivity or interference between human Cytochrome P450 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml - 2000 pg/ml. Sensitivity: The minimum detectable dose of human Cytochrome P450 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Cytochrome P450 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Cytochrome P450 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Cytochrome P450 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Cytochrome P450 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_625102.1
ncbi gb acc num :
NC_003888.3
ncbi pathways :
Aminobenzoate Degradation Pathway (8541); Fatty Acid Degradation Pathway (8475); Fatty Acid Degradation Pathway (296); Microbial Metabolism In Diverse Environments Pathway (179870); Tryptophan Metabolism Pathway (8507); Tryptophan Metabolism Pathway (332); Octane Oxidation Pathway (543485)
size4 :
10x96-Strip-Wells