ELISA/assay

Human Interleukin 24, IL-24 ELISA Kit

MBS705499

96 Strip Wells

760 USD

ELISA Kit

Human Interleukin 24, IL-24 ELISA Kit

interleukin 24

Human Interleukin 24 (IL-24) ELISA Kit; C49A; FISP; IL-24; IL10B; MDA7; Mob-5; ST16; mda-7; IL-24 splice variant delE3; IL-24 splice variant delE5; melanoma differentiation association protein 7; suppression of tumorigenicity 16 (melanoma di; interleukin 24

interleukin-24 isoform 3; Interleukin-24; interleukin-24; melanocyte-associated Mda-7; IL-4-induced secreted protein; melanoma differentiation-associated gene 7 protein; suppression of tumorigenicity 16 (melanoma differentiation); interleukin 24; Melanoma differentiation-associated gene 7 protein; MDA-7; Suppression of tumorigenicity 16 protein

IL24

IL24; IL24; C49A; FISP; MDA7; MOB5; ST16; IL10B; MDA7; ST16; IL-24; MDA-7

IL24_HUMAN

Human

207

This assay has high sensitivity and excellent specificity for detection of Human IL24. No significant cross-reactivity or interference between Human IL24 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, cell culture supernates. Detection Range: 15.6 pg/ml-1000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 3.9 pg/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Apoptosis/Autophagy. Protein Biological Process 3: Apoptosis

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to IL-24. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for IL-24 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain IL-24, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of IL-24 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

297632401

NP_001172085.1

NM_001185156.1

Q13007

23,825 Da

Cytokine-cytokine Receptor Interaction Pathway 83051!!Cytokine-cytokine Receptor Interaction Pathway 460!!IL23-mediated Signaling Events Pathway 138000!!Jak-STAT Signaling Pathway 83077!!Jak-STAT Signaling Pathway 488!!Senescence And Autophagy Pathway 198780

This gene encodes a member of the IL10 family of cytokines. It was identified as a gene induced during terminal differentiation in melanoma cells. The protein encoded by this gene can induce apoptosis selectively in various cancer cells. Overexpression of this gene leads to elevated expression of several GADD family genes, which correlates with the induction of apoptosis. The phosphorylation of mitogen-activated protein kinase 14 (MAPK7/P38), and heat shock 27kDa protein 1 (HSPB2/HSP27) are found to be induced by this gene in melanoma cells, but not in normal immortal melanocytes. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Function: Has antiproliferative properties on melanoma cells and may contribute to terminal cell differentiation.

96 Strip Wells

760 USD