catalog number :
MBS705496
products type :
ELISA Kit
products full name :
Pig prolactin, PRL ELISA Kit
products short name :
prolactin
products name syn :
Pig prolactin (PRL) ELISA Kit; prolactin
other names :
prolactin; Prolactin; prolactin
other gene names :
PRL; PRL; PRL
uniprot entry name :
PRL_PIG
specificity :
This assay has high sensitivity and excellent specificity for detection of pig PRL. No significant cross-reactivity or interference between pig PRL and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 0.78 pg/ml -50 pg/ml. Sensitivity: The minimum detectable dose of pig PRL is typically less than 0.195 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PRL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRL bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_999091.1
ncbi gb acc num :
NM_213926.1
ncbi mol weight :
26,141 Da
ncbi pathways :
Cytokine-cytokine Receptor Interaction Pathway (84463); Cytokine-cytokine Receptor Interaction Pathway (460); Jak-STAT Signaling Pathway (84489); Jak-STAT Signaling Pathway (488); Neuroactive Ligand-receptor Interaction Pathway (84465); Neuroactive Ligand-receptor Interaction Pathway (462); PI3K-Akt Signaling Pathway (692249); PI3K-Akt Signaling Pathway (692979); Prolactin Signaling Pathway (814206); Prolactin Signaling Pathway (814248)
uniprot summary :
Function: Prolactin acts primarily on the mammary gland by promoting lactation.
size4 :
10x96-Strip-Wells