Human Muscle Skeletal Receptor Tyrosine Kinase, MUSK ELISA Kit | MyBioSource MBS705494 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Muscle Skeletal Receptor Tyrosine Kinase, MUSK ELISA Kit

catalog :

MBS705494

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS705494

products type :

ELISA Kit

products full name :

Human Muscle Skeletal Receptor Tyrosine Kinase, MUSK ELISA Kit

products short name :

muscle, skeletal, receptor tyrosine kinase

products name syn :

Human Muscle Skeletal Receptor Tyrosine Kinase (MUSK) ELISA Kit; RP11-104M22.1; MGC126323; MGC126324; skeletal muscle receptor tyrosine kinase; muscle; skeletal; receptor tyrosine kinase

other names :

muscle, skeletal receptor tyrosine-protein kinase isoform 2; Muscle, skeletal receptor tyrosine-protein kinase; muscle, skeletal receptor tyrosine-protein kinase; muscle-specific kinase receptor; muscle-specific tyrosine-protein kinase receptor; muscle, skeletal, receptor tyrosine kinase; Muscle-specific tyrosine-protein kinase receptor; MuSK; Muscle-specific kinase receptor

products gene name :

MUSK

other gene names :

MUSK; MUSK; MuSK; Muscle-specific kinase receptor

uniprot entry name :

MUSK_HUMAN

reactivity :

Human

sequence length :

783

specificity :

This assay has high sensitivity and excellent specificity for detection of human MUSK. No significant cross-reactivity or interference between human MUSK and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 pg/ml -100 pg/ml. Sensitivity: The minimum detectable dose of human MUSK is typically less than 0.39 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for MUSK has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any MUSK present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MUSK is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MUSK bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

261878455

ncbi acc num :

NP_001159752.1

ncbi gb acc num :

NM_001166280.1

uniprot acc num :

O15146

ncbi mol weight :

97,056 Da

ncbi pathways :

ECM Proteoglycans Pathway (833812); Extracellular Matrix Organization Pathway (576262)

ncbi summary :

This gene encodes a muscle-specific tyrosine kinase receptor. The encoded protein may play a role in clustering of the acetylcholine receptor in the postsynaptic neuromuscular junction. Mutations in this gene have been associated with congenital myasthenic syndrome. Alternatively spliced transcript variants have been described.[provided by RefSeq, Oct 2009]

uniprot summary :

Function: Receptor tyrosine kinase which plays a central role in the formation and the maintenance of the neuromuscular junction (NMJ), the synapse between the motor neuron and the skeletal muscle. Recruitment of AGRIN by LRP4 to the MUSK signaling complex induces phosphorylation and activation of MUSK, the kinase of the complex. The activation of MUSK in myotubes regulates the formation of NMJs through the regulation of different processes including the specific expression of genes in subsynaptic nuclei, the reorganization of the actin cytoskeleton and the clustering of the acetylcholine receptors (AChR) in the postsynaptic membrane. May regulate AChR phosphorylation and clustering through activation of ABL1 and Src family kinases which in turn regulate MUSK. DVL1 and PAK1 that form a ternary complex with MUSK are also important for MUSK-dependent regulation of AChR clustering. May positively regulate Rho family GTPases through FNTA. Mediates the phosphorylation of FNTA which promotes prenylation, recruitment to membranes and activation of RAC1 a regulator of the actin cytoskeleton and of gene expression. Other effectors of the MUSK signaling include DNAJA3 which functions downstream of MUSK. May also play a role within the central nervous system by mediating cholinergic responses, synaptic plasticity and memory formation . Catalytic activity: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. Enzyme regulation: Positively regulated by CK2

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage