catalog number :
MBS705488
products type :
ELISA Kit
products full name :
Rat toll-like receptor 4, TLR4 ELISA Kit
products short name :
toll-like receptor 4
products name syn :
Rat toll-like receptor 4 (TLR4) ELISA Kit; ARMD10; CD284; TOLL; hToll; homolog of Drosophila toll; toll-like receptor 4
other names :
toll-like receptor 4; Toll-like receptor 4; toll-like receptor 4; toll4; toll-like receptor 4; CD_antigen: CD284
products gene name :
TLR4
other gene names :
Tlr4; Tlr4; Toll4
uniprot entry name :
TLR4_RAT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat TLR4. No significant cross-reactivity or interference between rat TLR4 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 0.156 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TLR4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TLR4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TLR4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TLR4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_062051.1
ncbi gb acc num :
NM_019178.1
ncbi mol weight :
96,072 Da
ncbi pathways :
Amoebiasis Pathway (167311); Amoebiasis Pathway (167191); Chagas Disease (American Trypanosomiasis) Pathway (147806); Chagas Disease (American Trypanosomiasis) Pathway (147795); HIF-1 Signaling Pathway (695233); Hepatitis B Pathway (694795); Inflammatory Bowel Disease (IBD) Pathway (842782); Inflammatory Bowel Disease (IBD) Pathway (842797); Influenza A Pathway (217170); Influenza A Pathway (217150)
ncbi summary :
receptor that functions as the major upstream sensor for hemorrhagic shock and lipopolysaccharide [RGD, Feb 2006]
uniprot summary :
Function: Cooperates with LY96 and CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MYD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response
size4 :
10x96-Strip-Wells