Human soluble Cluster of differentiation 100, sCD100 ELISA Kit | MyBioSource MBS705483 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human soluble Cluster of differentiation 100, sCD100 ELISA Kit

catalog :

MBS705483

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS705483

products type :

ELISA Kit

products full name :

Human soluble Cluster of differentiation 100, sCD100 ELISA Kit

products short name :

sema domain, immunoglobulin domain (Ig) , transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D

products name syn :

Human soluble Cluster of differentiation 100 (sCD100) ELISA kit; XXyac-YM68G7.1; C9orf164; CD100; FLJ33485; FLJ34282; FLJ39737; FLJ46484; M-sema-G; MGC169138; MGC169141; SEMAJ; coll-4; OTTHUMP00000021622; OTTHUMP00000208950; OTTHUMP00000208951; OTTHUMP00000208957; se; sema domain; immunoglobulin domain (Ig); transmembrane domain (TM) and short cytoplasmic domain; (semaphorin) 4D

other names :

semaphorin-4D isoform 2; Semaphorin-4D; semaphorin-4D; A8; GR3; BB18; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, 4D; sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4D; A8; BB18; GR3; CD_antigen: CD100

products gene name :

SEMA4D

other gene names :

SEMA4D; SEMA4D; CD100; SEMAJ; coll-4; C9orf164; M-sema-G; C9orf164; CD100; SEMAJ

uniprot entry name :

SEM4D_HUMAN

reactivity :

Human

sequence length :

738

specificity :

This assay has high sensitivity and excellent specificity for detection of human sCD100. No significant cross-reactivity or interference between human sCD100 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 125 pg/ml -8000 pg/ml. Sensitivity: 31.25 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for sCD100 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any sCD100 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for sCD100 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of sCD100 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

214010220

ncbi acc num :

NP_001135759.1

ncbi gb acc num :

NM_001142287.1

uniprot acc num :

Q92854

ncbi mol weight :

96,150 Da

ncbi pathways :

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); Other Semaphorin Interactions Pathway (119526); Sema4D In Semaphorin Signaling Pathway (119520); Sema4D Induced Cell Migration And Growth-cone Collapse Pathway (119522); Sema4D Mediated Inhibition Of Cell Attachment And Migration Pathway (119521); Semaphorin Interactions Pathway (119519)

uniprot summary :

Function: Cell surface receptor for PLXN1B and PLXNB2 that plays an important role in cell-cell signaling. Promotes reorganization of the actin cytoskeleton and plays a role in axonal growth cone guidance in the developing central nervous system. Regulates dendrite and axon branching and morphogenesis. Promotes the migration of cerebellar granule cells and of endothelial cells. Plays a role in the immune system; induces B-cells to aggregate and improves their viability (in vitro). Promotes signaling via SRC and PTK2B/PYK2, which then mediates activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Interaction with PLXNB1 mediates activation of RHOA.4 PublicationsManual assertion based on experiment in:Ref.1

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage