catalog number :
MBS705479
products type :
ELISA Kit
products full name :
Pig Lysyl Oxidase ELISA Kit
products short name :
lysyl oxidase
products name syn :
Pig Lysyl Oxidase ELISA Kit; MGC105112; protein-lysine 6-oxidase; lysyl oxidase
other names :
Protein-lysine 6-oxidase; Protein-lysine 6-oxidase; Lysyl oxidase
uniprot entry name :
LYOX_PIG
specificity :
This assay has high sensitivity and excellent specificity for detection of pig lysyl oxidase. No significant cross-reactivity or interference between pig lysyl oxidase and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma and tissue homogenates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml -100 ng/ml. Sensitivity: The minimum detectable dose of pig lysyl oxidase is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for lysyl oxidase has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any lysyl oxidase present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for lysyl oxidase is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of lysyl oxidase bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
29,073 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
uniprot summary :
Function: Responsible for the post-translational oxidative deamination of peptidyl lysine residues in precursors to fibrous collagen and elastin. Catalytic activity: Peptidyl-L-lysyl-peptide + O2 + H2O = peptidyl-allysyl-peptide + NH3 + H2O2. Cofactor: Copper
size4 :
10x96-Strip-Wells