ELISA/assay

Human B-cell leukemia/lymphoma 6, Bcl6 ELISA Kit

MBS705459

48-Strip-Wells

565 USD

ELISA Kit

Human B-cell leukemia/lymphoma 6, Bcl6 ELISA Kit

B-cell CLL/lymphoma 6

Human B-cell leukemia/lymphoma 6; Bcl6 ELISA Kit; BCL5; BCL6A; LAZ3; ZBTB27; ZNF51; B-cell lymphoma 6 protein; B-cell lymphoma 6 protein transcript; cys-his2 zinc finger transcription factor; lymphoma-associated zinc finger gene on chromosome 3; zinc f; B-cell CLL/lymphoma 6

B-cell lymphoma 6 protein isoform 1; B-cell lymphoma 6 protein; B-cell lymphoma 6 protein; BCL-5; BCL-6; protein LAZ-3; zinc finger protein 51; B-cell lymphoma 5 protein; B-cell lymphoma 6 protein transcript; zinc finger transcription factor BCL6S; cys-his2 zinc finger transcription factor; zinc finger and BTB domain-containing protein 27; lymphoma-associated zinc finger gene on chromosome 3; B-cell CLL/lymphoma 6; B-cell lymphoma 5 protein; BCL-5; Protein LAZ-3; Zinc finger and BTB domain-containing protein 27; Zinc finger protein 51

BCL6

BCL6; BCL6; BCL5; LAZ3; BCL6A; ZNF51; ZBTB27; BCL5; LAZ3; ZBTB27; ZNF51; BCL-6; BCL-5

BCL6_HUMAN

Human

706

This assay has high sensitivity and excellent specificity for detection of human Bcl6. No significant cross-reactivity or interference between human Bcl6 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml -1200 pg/ml. Sensitivity: The minimum detectable dose of human Bcl6 is typically less than 4.67 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for Bcl6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any Bcl6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for Bcl6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Bcl6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

195927002

NP_001124317.1

NM_001130845.1

P41182

78,846 Da

B Cell Receptor Signaling Pathway (198909); DNA Damage Response (only ATM Dependent) Pathway (198827); Direct P53 Effectors Pathway (137939); FoxO Family Signaling Pathway (138036); FoxO Signaling Pathway (921162); IL4-mediated Signaling Events Pathway (137933); Signaling Events Mediated By HDAC Class II Pathway (138062); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987)

The protein encoded by this gene is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of START-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. This gene is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

Function: Transcriptional repressor mainly required for germinal center (GC) formation and antibody affinity maturation which has different mechanisms of action specific to the lineage and biological functions. Forms complexes with different corepressors and histone deacetylases to repress the transcriptional expression of different subsets of target genes. Represses its target genes by binding directly to the DNA sequence 5'-TTCCTAGAA-3' (BCL6-binding site) or indirectly by repressing the transcriptional activity of transcription factors. In GC B-cells, represses genes that function in differentiation, inflammation, apoptosis and cell cycle control, also autoregulates its transcriptional expression and up-regulates, indirectly, the expression of some genes important for GC reactions, such as AICDA, through the repression of microRNAs expression, like miR155. An important function is to allow GC B-cells to proliferate very rapidly in response to T-cell dependent antigens and tolerate the physiological DNA breaks required for immunglobulin class switch recombination and somatic hypermutation without inducing a p53/TP53-dependent apoptotic response. In follicular helper CD4+ T-cells (T(FH) cells), promotes the expression of T(FH)-related genes but inhibits the differentiation of T(H)1, T(H)2 and T(H)17 cells. Also required for the establishment and maintenance of immunological memory for both T- and B-cells. Suppresses macrophage proliferation through competition with STAT5 for STAT-binding motifs binding on certain target genes, such as CCL2 and CCND2. In response to genotoxic stress, controls cell cycle arrest in GC B-cells in both p53/TP53-dependedent and -independent manners. Besides, also controls neurogenesis through the alteration of the composition of NOTCH-dependent transcriptional complexes at selective NOTCH targets, such as HES5, including the recruitment of the deacetylase SIRT1 and resulting in an epigenetic silencing leading to neuronal differentiation.14 PublicationsManual assertion based on experiment in:Ref.9

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150