catalog number :
MBS705437
products type :
ELISA Kit
products full name :
Rat 6-keto-PGF1alpha ELISA Kit
products short name :
[6-keto-PGF1alpha]
products name syn :
[Rat 6-keto-PGF1alpha ELISA Kit; 6-keto-PGF1alpha]
products gene name :
[6-keto-PGF1alpha]
specificity :
This assay has high sensitivity and excellent specificity for detection of human 5-HETE. No significant cross-reactivity or interference between human 5-HETE and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
image1 heading :
Typical Testing Data/Standard Curve (for reference only)
other info1 :
Samples: Serum, Plasma, Cell Culture Supernates. Assay Type: Quantitative Sandwich. Detection Range: 31.25 pg/ml-2000 pg/ml. Sensitivity: 7.81 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for 5-HETE has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any 5-HETE present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for 5-HETE is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of 5-HETE bound in the initial step. The color development is stopped and the intensity of the color is measured.
size1 :
24-Strip-Wells (LIMIT 1)
size5 :
10x96-Strip-Wells