Human B7-H4 ELISA Kit | MyBioSource MBS705429 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human B7-H4 ELISA Kit

catalog :

MBS705429

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS705429

products type :

ELISA Kit

products full name :

Human B7-H4 ELISA Kit

products short name :

V-set domain containing T cell activation inhibitor 1

products name syn :

Human B7-H4 ELISA Kit; B7-H4; B7H4; B7S1; B7X; B7h.5; FLJ22418; PRO1291; RP11-229A19.4; VCTN1; T cell costimulatory molecule B7x; immune costimulatory protein B7-H4; V-set domain containing T cell activation inhibitor 1

other names :

V-set domain-containing T-cell activation inhibitor 1 isoform 2; V-set domain-containing T-cell activation inhibitor 1; V-set domain-containing T-cell activation inhibitor 1; B7 family member, H4; B7 superfamily member 1; T cell costimulatory molecule B7x; T-cell costimulatory molecule B7x; immune costimulatory protein B7-H4; V-set domain containing T cell activation inhibitor 1; B7h.5; Immune costimulatory protein B7-H4; Protein B7S1; T-cell costimulatory molecule B7x

products gene name :

VTCN1

other gene names :

VTCN1; VTCN1; B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291; B7H4; UNQ659/PRO1291

uniprot entry name :

VTCN1_HUMAN

reactivity :

Human

sequence length :

187

specificity :

This assay has high sensitivity and excellent specificity for detection of human B7-H4. No significant cross-reactivity or interference between human B7-H4 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates and tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 ng/ml -500 ng/ml. Sensitivity: The minimum detectable dose of human B7-H4 is typically less than 1.95 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for B7-H4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any B7-H4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for B7-H4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of B7-H4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

359718944

ncbi acc num :

NP_001240778.1

ncbi gb acc num :

NM_001253849.1

uniprot acc num :

Q7Z7D3

ncbi mol weight :

30,878 Da

ncbi pathways :

Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480)

ncbi summary :

This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]

uniprot summary :

Function: Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.By similarityManual assertion inferred from sequence similarityUniProtKB:Q7TSP5

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage