catalog number :
MBS705422
products type :
ELISA Kit
products full name :
Rat Pro-opiomelanocortin, POMC ELISA Kit
products short name :
proopiomelanocortin
products name syn :
Rat Pro-opiomelanocortin (POMC) ELISA KIT; ACTH; CLIP; LPH; MSH; NPP; POC; OTTHUMP00000119991; OTTHUMP00000200964; adrenocorticotropic hormone; adrenocorticotropin; alpha-MSH; alpha-melanocyte-stimulating hormone; beta-LPH; beta-MSH; beta-endorphin; ; proopiomelanocortin
other names :
Pro-opiomelanocortin; Pro-opiomelanocortin; Corticotropin-lipotropinCleaved into the following 3 chains:CorticotropinAlternative name(s):Adrenocorticotropic hormone; ACTH
products gene name :
POMC
other gene names :
POMC; POMC; ACTH; CLIP
uniprot entry name :
COLI_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat POMC. No significant cross-reactivity or interference between Rat POMC and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml-500 pg/ml. Sensitivity: 1.95 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for POMC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any POMC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POMC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POMC bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi mol weight :
4,478 Da
ncbi pathways :
Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Androgen Biosynthesis Pathway (106154); Biological Oxidations Pathway (105698); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Cytochrome P450 - Arranged By Substrate Type Pathway (105700); Endogenous Sterols Pathway (105701); G Alpha (i) Signalling Events Pathway (119550); G Alpha (s) Signalling Events Pathway (119549); G-protein Activation Pathway (106494)
uniprot summary :
Function: ACTH stimulates the adrenal glands to release cortisol.
size4 :
10x96-Strip-Wells