Human Heat Shock Protein 72, HSP-72 ELISA Kit | MyBioSource MBS705388 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Heat Shock Protein 72, HSP-72 ELISA Kit

catalog :

MBS705388

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS705388

products type :

ELISA Kit

products full name :

Human Heat Shock Protein 72, HSP-72 ELISA Kit

products short name :

Heat Shock Protein 72 (HSP-72)

products name syn :

Human Heat Shock Protein 72 (HSP-72) ELISA Kit; Heat Shock Protein 72 (HSP-72)

other names :

heat shock protein 72, partial; Heat shock 70 kDa protein 1A/1B; heat shock 70 kDa protein 1A/1B; HSP70-1/HSP70-2; heat shock 70kD protein 1A; heat shock-induced protein; heat shock 70 kDa protein 1/2; epididymis secretory protein Li 103; dnaK-type molecular chaperone HSP70-1; heat shock 70kDa protein 1A; Heat shock 70 kDa protein 1/2; HSP70-1/HSP70-2; HSP70.1/HSP70.2

products gene name :

HSP-72

other gene names :

HSPA1A; HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70-1A; HEL-S-103; HSPA1; HSX70 HSPA1B ; HSP70-1/HSP70-2; HSP70.1/HSP70.2

uniprot entry name :

HSP71_HUMAN

reactivity :

Human

sequence length :

151

specificity :

This assay has high sensitivity and excellent specificity for detection of human HSP-72. No significant cross-reactivity or interference between human HSP-72 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, cell lysates. Assay Type: Sandwich. Detection Range: 3.12 ng/ml -200 ng/ml. Sensitivity: The minimum detectable dose of human HSP-72 is typically less than 0.78 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HSP-72 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HSP-72 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HSP-72 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HSP-72 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4691418

ncbi acc num :

BAA77235.1

uniprot acc num :

P08107

ncbi mol weight :

63,937 Da

ncbi pathways :

Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Apoptosis Modulation By HSP70 Pathway (198877); Direct P53 Effectors Pathway (137939); Endocytosis Pathway (102279); Endocytosis Pathway (102181); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); Estrogen Signaling Pathway (799177); Estrogen Signaling Pathway (799197)

ncbi summary :

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins. [provided by RefSeq, Jul 2008]

uniprot summary :

Function: In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. In case of rotavirus A infection, serves as a post-attachment receptor for the virus to facilitate entry into the cell.1 PublicationManual assertion based on experiment in:Ref.20

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage