ELISA/assay

Rat Creatine Kinase MB isoenzyme, CK-MB ELISA Kit

MBS705376

48-Strip-Wells

510 USD

ELISA Kit

Rat Creatine Kinase MB isoenzyme, CK-MB ELISA Kit

Creatine Kinase MB isoenzyme, CK-MB

Rat Creatine Kinase MB isoenzyme; CK-MB ELISA Kit; Creatine Kinase MB isoenzyme; CK-MB

creatine kinase M-type; Creatine kinase M-type; creatine kinase M-type; creatine kinase M chain; creatine kinase, muscle; Creatine kinase M chain; M-CKCleaved into the following chain:Creatine kinase M-type, N-terminally processed

CK-MB

CKM; CKM; CKMM; M-CK; CKMM

KCRM_HUMAN

Rat

381

This assay has high sensitivity and excellent specificity for detection of rat CK-MB. No significant cross-reactivity or interference between rat CK-MB and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, urine, tissue homogenates and plasma. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 0.078 ng/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CK-MB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CK-MB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CK-MB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CK-MB bound in the initial step. The color development is stopped and the intensity of the color is measured.

21536288

NP_001815.2

NM_001824.4

P06732

43,101 Da

Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Creatine Metabolism Pathway (106192); Creatine Pathway (413359); Creatine Pathway (468234); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Regulation Of Retinoblastoma Protein Pathway (137916); Urea Cycle And Metabolism Of Amino Groups Pathway (198758)

The protein encoded by this gene is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. The encoded protein reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. The encoded protein is a member of the ATP:guanido phosphotransferase protein family. [provided by RefSeq, Jul 2008]

Function: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Catalytic activity: ATP + creatine = ADP + phosphocreatine.

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800