catalog number :
MBS705362
products type :
ELISA Kit
products full name :
Human Agmatine Ureohydrolase/Agmatinase, AGMAT ELISA Kit
products short name :
agmatine ureohydrolase (agmatinase)
products name syn :
Human Agmatine Ureohydrolase/Agmatinase (AGMAT) ELISA Kit; FLJ23384; agmatinase; agmatine ureohydrolase (agmatinase)
other names :
agmatinase, mitochondrial; Agmatinase, mitochondrial; agmatinase, mitochondrial; AUH; agmatine ureohydrolase (agmatinase); Agmatine ureohydrolase; AUH
products gene name :
AGMAT
other gene names :
AGMAT; AGMAT; AUH
uniprot entry name :
SPEB_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human AGMAT. No significant cross-reactivity or interference between human AGMAT and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 39 pg/ml -2500 pg/ml. Sensitivity: 9.75 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AGMAT has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AGMAT present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AGMAT is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AGMAT bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_079034.3
ncbi gb acc num :
NM_024758.4
ncbi mol weight :
37,660 Da
ncbi pathways :
Agmatine Biosynthesis Pathway (106194); Arginine And Proline Metabolism Pathway (82957); Arginine And Proline Metabolism Pathway (323); Metabolic Pathways (132956); Metabolism Pathway (477135); Metabolism Of Amino Acids And Derivatives Pathway (106169); Metabolism Of Polyamines Pathway (106193); Polyamine Biosynthesis, Arginine = Agmatine = Putrescine = Spermidine Pathway (413402); Polyamine Biosynthesis, Arginine = Agmatine = Putrescine = Spermidine Pathway (468310)
uniprot summary :
AGMAT: Belongs to the arginase family. Agmatinase subfamily. Protein type: EC 3.5.3.11; Amino Acid Metabolism - arginine and proline; Hydrolase. Chromosomal Location of Human Ortholog: 1p36.21. Cellular Component: mitochondrion. Molecular Function: metal ion binding; agmatinase activity. Biological Process: putrescine biosynthetic process from arginine; spermidine biosynthetic process; polyamine metabolic process
size4 :
10x96-Strip-Wells