product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Monkey Leptin, LEP ELISA Kit
catalog :
MBS705354
quantity :
48-Strip-Wells
price :
490 USD
more info or order :
citations: 1
Reference
Polewski M, Burhans M, Zhao M, Colman R, Shanmuganayagam D, Lindstrom M, et al. Plasma diacylglycerol composition is a biomarker of metabolic syndrome onset in rhesus monkeys. J Lipid Res. 2015;56:1461-70 pubmed publisher
product information
catalog number :
MBS705354
products type :
ELISA Kit
products full name :
Monkey Leptin, LEP ELISA Kit
products short name :
leptin
products name syn :
Monkey Leptin; LEP ELISA Kit; FLJ94114; OB; OBS; leptin (murine obesity homolog) ; leptin (obesity homolog; mouse) ; obese; mouse; homolog of; obesity factor; leptin
other names :
leptin; Leptin; leptin; obesity factor; Obesity factor
products gene name :
LEP
other gene names :
LEP; LEP; OB; OB
uniprot entry name :
LEP_MACMU
reactivity :
Monkey
sequence length :
167
specificity :
This assay has high sensitivity and excellent specificity for detection of monkey leptin. No significant cross-reactivity or interference between monkey leptin and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma and tissue homogenates. Assay Type: Competitive. Detection Range: 3.125 ng/ml -50 ng/ml. Sensitivity: 3.125 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to leptin. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated leptin. A competitive inhibition reaction is launched between leptin (Standards or samples) and Biotin-conjugated leptin with the pre-coated antibody specific for leptin. The more amount of leptin in samples, the less antibody bound by Biotin-conjugated leptin. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of leptin in the sample. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
112363109
ncbi acc num :
NP_001036220.1
ncbi gb acc num :
NM_001042755.1
uniprot acc num :
Q28504
ncbi mol weight :
18,953 Da
ncbi pathways :
AMPK Signaling Pathway (989602); AMPK Signaling Pathway (992181); Adipocytokine Signaling Pathway (86763); Adipocytokine Signaling Pathway (505); Cytokine-cytokine Receptor Interaction Pathway (86721); Cytokine-cytokine Receptor Interaction Pathway (460); Jak-STAT Signaling Pathway (86747); Jak-STAT Signaling Pathway (488); Neuroactive Ligand-receptor Interaction Pathway (86723); Neuroactive Ligand-receptor Interaction Pathway (462)
uniprot summary :
Function: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
size1 :
48-Strip-Wells
price1 :
490 USD
size2 :
96-Strip-Wells
price2 :
690
size3 :
5x96-Strip-Wells
price3 :
2560
size4 :
10x96-Strip-Wells
price4 :
4790
more info or order :
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

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