catalog number :
MBS705354
products type :
ELISA Kit
products full name :
Monkey Leptin, LEP ELISA Kit
products short name :
leptin
products name syn :
Monkey Leptin; LEP ELISA Kit; FLJ94114; OB; OBS; leptin (murine obesity homolog) ; leptin (obesity homolog; mouse) ; obese; mouse; homolog of; obesity factor; leptin
other names :
leptin; Leptin; leptin; obesity factor; Obesity factor
other gene names :
LEP; LEP; OB; OB
uniprot entry name :
LEP_MACMU
specificity :
This assay has high sensitivity and excellent specificity for detection of monkey leptin. No significant cross-reactivity or interference between monkey leptin and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma and tissue homogenates. Assay Type: Competitive. Detection Range: 3.125 ng/ml -50 ng/ml. Sensitivity: 3.125 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<15%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<15%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to leptin. Standards or samples are added to the appropriate microtiter plate wells with Biotin-conjugated leptin. A competitive inhibition reaction is launched between leptin (Standards or samples) and Biotin-conjugated leptin with the pre-coated antibody specific for leptin. The more amount of leptin in samples, the less antibody bound by Biotin-conjugated leptin. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Substrate solution is added to the wells and the color develops in opposite to the amount of leptin in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001036220.1
ncbi gb acc num :
NM_001042755.1
ncbi mol weight :
18,953 Da
ncbi pathways :
AMPK Signaling Pathway (989602); AMPK Signaling Pathway (992181); Adipocytokine Signaling Pathway (86763); Adipocytokine Signaling Pathway (505); Cytokine-cytokine Receptor Interaction Pathway (86721); Cytokine-cytokine Receptor Interaction Pathway (460); Jak-STAT Signaling Pathway (86747); Jak-STAT Signaling Pathway (488); Neuroactive Ligand-receptor Interaction Pathway (86723); Neuroactive Ligand-receptor Interaction Pathway (462)
uniprot summary :
Function: May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass.
size4 :
10x96-Strip-Wells