catalog number :
MBS705348
products type :
ELISA Kit
products full name :
Human Spondin-2 ELISA Kit
products short name :
spondin 2, extracellular matrix protein
products name syn :
Human Spondin-2 ELISA Kit; DIL-1; DIL1; DKFZp686G21139; FLJ16313; FLJ34460; M-spondin; Mindin; ; spondin 2; extracellular matrix protein
other names :
spondin-2; Spondin-2; spondin-2; differentially expressed in cancerous and non-cancerous lung cells 1; spondin 2, extracellular matrix protein; Differentially expressed in cancerous and non-cancerous lung cells 1; DIL-1; Mindin
products gene name :
SPON2
other gene names :
SPON2; SPON2; DIL1; DIL-1; MINDIN; M-SPONDIN; DIL1; UNQ435/PRO866; DIL-1
uniprot entry name :
SPON2_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human spondin-2. No significant cross-reactivity or interference between human spondin-2 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cerebrospinal fluid (CSF), cell culture supernates. Assay Type: Sandwich. Detection Range: 3.12 ng/ml -200 ng/ml. Sensitivity: 0.78 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for spondin-2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any spondin-2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for spondin-2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of spondin-2 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001121797.1
ncbi gb acc num :
NM_001128325.2
ncbi mol weight :
35,846 Da
ncbi pathways :
Metabolism Of Proteins Pathway (106230); O-glycosylation Of TSR Domain-containing Proteins Pathway (980460); O-linked Glycosylation Pathway (980459); Post-translational Protein Modification Pathway (161012)
uniprot summary :
Function: Cell adhesion protein that promotes adhesion and outgrowth of hippocampal embryonic neurons. Binds directly to bacteria and their components and functions as an opsonin for macrophage phagocytosis of bacteria. Essential in the initiation of the innate immune response and represents a unique pattern-recognition molecule in the ECM for microbial pathogens Binds bacterial lipopolysaccharide (LPS).
size4 :
10x96-Strip-Wells