catalog number :
MBS705342
products type :
ELISA Kit
products full name :
Dog growth hormone, GH ELISA Kit
products short name :
growth hormone 1
products name syn :
Dog growth hormone; GH ELISA Kit; GH; GH-N; GHN; IGHD1B; hGH-N; pituitary growth hormone; growth hormone 1
other names :
somatotropin; Somatotropin; somatotropin; Growth hormone
other gene names :
GH1; GH1; GH; GH
uniprot entry name :
SOMA_CANFA
specificity :
This assay has high sensitivity and excellent specificity for detection of Dog GH1. No significant cross-reactivity or interference between Dog GH1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma and tissue homogenates. Assay Type: Competitive. Detection Range: 12.5 ng/ml-200 ng/ml. Sensitivity: 2.5 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to GH has been pre-coated onto a microplate. Standards or samples are added to the appropriate microtiter plate wells with biotin-conjugated GH and incubated. A competitive inhibition reaction is launched between GH (Standards or samples) and Biotin-conjugated GH with the pre-coated antibody specific for GH. The more amount of GH in samples, the less antibody bound by Biotin-conjugated GH. Then Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The substrate solutions are added to the wells, respectively. And the color develops in opposite to the amount of GH in the sample. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001003168.1
ncbi gb acc num :
NM_001003168.1
ncbi mol weight :
24,468 Da
ncbi pathways :
Cytokine Signaling In Immune System Pathway (1016476); Growth Hormone Receptor Signaling Pathway (1016493); Immune System Pathway (1016347); Jak-STAT Signaling Pathway (84127); Jak-STAT Signaling Pathway (488); Metabolism Of Proteins Pathway (1016851); Neuroactive Ligand-receptor Interaction Pathway (84103); Neuroactive Ligand-receptor Interaction Pathway (462); Nuclear Signaling By ERBB4 Pathway (1017173); PI3K-Akt Signaling Pathway (692237)
uniprot summary :
Function: Plays an important role in growth control. Its major role in stimulating body growth is to stimulate the liver and other tissues to secrete IGF-1. It stimulates both the differentiation and proliferation of myoblasts. It also stimulates amino acid uptake and protein synthesis in muscle and other tissues.
size4 :
10x96-Strip-Wells