catalog number :
MBS705333
products type :
ELISA Kit
products full name :
Mouse low density lipoprotein, LDL ELISA Kit
products short name :
low density lipoprotein, LDL
products name syn :
Mouse low density lipoprotein; LDL ELISA Kit; low density lipoprotein; LDL
other names :
L-lactate dehydrogenase, partial; L-lactate dehydrogenase; L-lactate dehydrogenase
uniprot entry name :
G3G1T6_BIFLN
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse LDL. No significant cross-reactivity or interference between mouse LDL and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Competitive. Detection Range: 7.8 ng/ml -500 ng/ml. Sensitivity: The minimum detectable dose of mouse LDL is typically less than 1.95 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with LDL. Standards or samples are added to the appropriate microtiter plate wells with Horseradish Peroxidase (HRP) conjugated antibody preparation specific for LDL. The competitive inhibition reaction is launched between with pre-coated LDL and LDL in samples. A substrate solution is added to the wells and the color develops in opposite to the amount of LDL in the samples. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
AEO91407.1
ncbi mol weight :
14,640 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size4 :
10x96-Strip-Wells