catalog number :
MBS705329
products type :
ELISA Kit
products full name :
New Delhi metallo-beta-lactamase-1, NDM-1 ELISA Kit
products short name :
New Delhi metallo-beta-lactamase-1 (NDM-1)
products name syn :
New Delhi metallo-beta-lactamase-1 (NDM-1) ELISA kit; New Delhi metallo-beta-lactamase-1 (NDM-1)
other names :
New Delhi metallo-beta-lactamase-1; Beta-lactamase NDM-1; Beta-lactamase NDM-1; Class B carbapenemase NDM-1; Metallo-beta-lactamase; Metallo-beta-lactamase 1; Metallo-beta-lactamase NDM-1; Metallo-beta-lactamase-1; NDM-1; New Delhi metal-beta-lactamase enzyme; New Delhi metallo-beta-lactamase-1
products gene name :
NDM-1
other gene names :
NDM-1; blaNDM-1; pNDM-AB_009
uniprot entry name :
F8UNN7_ACIBA
specificity :
This assay has high sensitivity and excellent specificity for detection of NDM-1. No significant cross-reactivity or interference between NDM-1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma and urine samples infected by New Delhi super bacteria. Assay Type: Sandwich. Detection Range: 6.25 ng/ml -400 ng/ml. Sensitivity: 3.12 ng /ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NDM-1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NDM-1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NDM-1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NDM-1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
AEJ10056.1
ncbi mol weight :
28,499 Da
ncbi pathways :
Focal Adhesion Pathway (83067); Focal Adhesion Pathway (478); MAPK Signaling Pathway (83048); MAPK Signaling Pathway (456); Salmonella Infection Pathway (375172); Salmonella Infection Pathway (375149)
size4 :
10x96-Strip-Wells