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company name :
MyBioSource
product type :
ELISA/assay
product name :
Rat Serotonin Receptor 2A/5-hydroxytryptamine Receptor 2A, 5-HT-2A ELISA Kit
catalog :
MBS705328
quantity :
96 Strip Wells
price :
850 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS705328
products type :
ELISA Kit
products full name :
Rat Serotonin Receptor 2A/5-hydroxytryptamine Receptor 2A, 5-HT-2A ELISA Kit
products short name :
5-hydroxytryptamine (serotonin) receptor 2A
products name syn :
Rat Serotonin Receptor 2A/5-hydroxytryptamine Receptor 2A (5-HT-2A) ELISA Kit; RP11-147L20.2; 5-HT2A; HTR2; 5-HT2 receptor; 5-hydroxytryptamine receptor 2A; serotonin 5-HT-2A receptor; 5-hydroxytryptamine (serotonin) receptor 2A
other names :
5-hydroxytryptamine receptor 2A; 5-hydroxytryptamine receptor 2A; 5-hydroxytryptamine receptor 2A; 5-HT-2; 5-HT-2A; serotonin receptor 2A; serotonin 5HT-2 receptor; 5-hydroxytryptamine (serotonin) receptor 2A, G protein-coupled; Serotonin receptor 2A
products gene name :
HTR2A
other gene names :
Htr2a; Htr2a; 5Ht-2; 5-HT2A; Htr2; 5-HT-2; 5-HT-2A
uniprot entry name :
5HT2A_RAT
reactivity :
Rat
sequence length :
471
specificity :
This assay has high sensitivity and excellent specificity for detection of Rat HTR2A. No significant cross-reactivity or interference between Rat HTR2A and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates, cell lysates. Detection Range: 31.25 pg/ml-2000 pg/ml. Detection Wavelength: 450 nm. Sensitivity: 7.8 pg/ml
other info2 :
Sample Volume: 50-100ul
products description :
Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to 5-HT-2A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for 5-HT-2A and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain 5-HT-2A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of 5-HT-2A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ncbi gi num :
8393583
ncbi acc num :
NP_058950.1
ncbi gb acc num :
NM_017254.1
uniprot acc num :
P14842
ncbi mol weight :
52,850 Da
ncbi pathways :
Amine Ligand-binding Receptors Pathway 1010172!!Calcium Signaling Pathway 83442!!Calcium Signaling Pathway 459!!Class A/1 (Rhodopsin-like Receptors) Pathway 1010164!!G Alpha (q) Signalling Events Pathway 1010197!!GPCR Downstream Signaling Pathway 1010193!!GPCR Ligand Binding Pathway 1010163!!GPCRs, Class A Rhodopsin-like Pathway 198500!!GPCRs, Other Pathway 198454!!Gap Junction Pathway 83464
ncbi summary :
a receptor for 5-hydroxytryptamine (serotonin); activates phospholipase C signaling pathway; involved in promoting cellular transformation [RGD, Feb 2006]
uniprot summary :
Function: G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances, including mescaline, psilocybin, 1-(2,5-dimethoxy-4-iodophenyl)-2-aminopropane (DOI) and lysergic acid diethylamide (LSD). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling activates phospholipase C and a phosphatidylinositol-calcium second messenger system that modulates the activity of phosphatidylinositol 3-kinase and promotes the release of Ca2+ ions from intracellular stores. Affects neural activity, perception, cognition and mood. Plays a role in the regulation of behavior, including responses to anxiogenic situations and psychoactive substances. Plays a role in intestinal smooth muscle contraction, and may play a role in arterial vasoconstriction.2 PublicationsManual assertion based on experiment in:Ref.1
size :
96 Strip Wells
price :
850 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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