Human phospholipase A2 receptor 1, 180kDa, PLA2R1 ELISA Kit | MyBioSource MBS705318 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human phospholipase A2 receptor 1, 180kDa, PLA2R1 ELISA Kit

catalog :

MBS705318

quantity :

48-Strip-Wells

price :

565 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS705318

products type :

ELISA Kit

products full name :

Human phospholipase A2 receptor 1, 180kDa, PLA2R1 ELISA Kit

products short name :

phospholipase A2 receptor 1, 180kDa

products name syn :

Human phospholipase A2 receptor 1; 180kDa (PLA2R1) ELISA kit; CLEC13C; PLA2-R; PLA2G1R; PLA2IR; PLA2R; phospholipase A2 receptor 1; phospholipase A2 receptor 1; 180kDa

other names :

secretory phospholipase A2 receptor isoform 2; Secretory phospholipase A2 receptor; secretory phospholipase A2 receptor; M-type receptor; phospholipase A2 receptor 1, 180kD; C-type lectin domain family 13 member C; 180 kDa secretory phospholipase A2 receptor; phospholipase A2 receptor 1, 180kDa; 180 kDa secretory phospholipase A2 receptor; C-type lectin domain family 13 member C; M-type receptorCleaved into the following chain:Soluble secretory phospholipase A2 receptor; Soluble PLA2-R; Soluble PLA2R

products gene name :

PLA2R1

other gene names :

PLA2R1; PLA2R1; PLA2R; PLA2-R; PLA2IR; CLEC13C; PLA2G1R; CLEC13C; PLA2-R; PLA2R; Soluble PLA2-R; Soluble PLA2R

uniprot entry name :

PLA2R_HUMAN

reactivity :

Human

sequence length :

1324

specificity :

This assay has high sensitivity and excellent specificity for detection of human PLA2R1. No significant cross-reactivity or interference between human PLA2R1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell culture supernates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.078 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLA2R1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLA2R1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLA2R1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLA2R1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

55953106

ncbi acc num :

NP_001007268.1

ncbi gb acc num :

NM_001007267.2

uniprot acc num :

Q13018

ncbi mol weight :

168,600 Da

ncbi pathways :

Phagosome Pathway (153910); Phagosome Pathway (153859); Tuberculosis Pathway (213780); Tuberculosis Pathway (213743)

ncbi summary :

This gene represents a phospholipase A2 receptor. The encoded protein likely exists as both a transmembrane form and a soluble form. The transmembrane receptor may play a role in clearance of phospholipase A2, thereby inhibiting its action. Polymorphisms at this locus have been associated with susceptibility to idiopathic membranous nephropathy. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Sep 2010]

uniprot summary :

Function: Receptor for secretory phospholipase A2 (sPLA2). Acts as a receptor for phosholipase sPLA2-IB/PLA2G1B but not sPLA2-IIA/PLA2G2A. Also able to bind to snake PA2-like toxins. Although its precise function remains unclear, binding of sPLA2 to its receptor participates in both positive and negative regulation of sPLA2 functions as well as clearance of sPLA2. Binding of sPLA2-IB/PLA2G1B induces various effects depending on the cell type, such as activation of the mitogen-activated protein kinase (MAPK) cascade to induce cell proliferation, the production of lipid mediators, selective release of arachidonic acid in bone marrow-derived mast cells. In neutrophils, binding of sPLA2-IB/PLA2G1B can activate p38 MAPK to stimulate elastase release and cell adhesion. May be involved in responses in proinflammatory cytokine productions during endotoxic shock. Also has endocytic properties and rapidly internalizes sPLA2 ligands, which is particularly important for the clearance of extracellular sPLA2s to protect their potent enzymatic activities. The soluble secretory phospholipase A2 receptor form is circulating and acts as a negative regulator of sPLA2 functions by blocking the biological functions of sPLA2-IB/PLA2G1B.2 PublicationsManual assertion based on experiment in:Ref.1

size1 :

48-Strip-Wells

price1 :

565 USD

size2 :

96-Strip-Wells

price2 :

810

size3 :

5x96-Strip-Wells

price3 :

2750

size4 :

10x96-Strip-Wells

price4 :

5150

more info or order :

MyBioSource product webpage