ELISA/assay

Rat Vitamin D-binding protein, DBP ELISA Kit

MBS705311

96 Strip Wells

850 USD

ELISA Kit

Rat Vitamin D-binding protein, DBP ELISA Kit

group-specific component (vitamin D binding protein)

Rat Vitamin D-binding protein (DBP) ELISA Kit; DBP; DBP/GC; GRD3; VDBG; VDBP; vitamin D-binding alpha-globulin; vitamin D-binding protein; group-specific component (vitamin D binding protein)

vitamin D-binding protein; Vitamin D-binding protein; vitamin D-binding protein; VDB; gc-globulin; D site of albumin promoter (albumin D-box) binding protein; Gc-globulin; Group-specific component

GC

GC; GC; DBP; DBP; DBP; VDB

VTDB_RABIT

Rat

476

This assay has high sensitivity and excellent specificity for detection of Rat GC. No significant cross-reactivity or interference between Rat GC and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Sample Type: serum, plasma, tissue homogenates. Detection Range: 0.18 ug/ml-12 ug/ml. Detection Wavelength: 450 nm. Sensitivity: 0.045 ug/ml

Sample Volume: 50-100ul. Protein Biological Process 1: Transport. Protein Biological Process 3: Transport

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DBP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for DBP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain DBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of DBP in the samples is then determined by comparing the O.D. of the samples to the standard curve.

126723668

NP_001075673.1

NM_001082204.1

P53789

52,912 Da

Function: Multifunctional protein found in plasma, ascitic fluid, cerebrospinal fluid, and urine and on the surface of many cell types. In plasma, it carries the vitamin D sterols and prevents polymerization of actin by binding its monomers. DBP associates with membrane-bound immunoglobulin on the surface of B-lymphocytes and with IgG Fc receptor on the membranes of T-lymphocytes.

96 Strip Wells

850 USD