ELISA/assay

Human Signal Peptide, CUB and EGF-like Domain-containing Protein 1, SCUBE1 ELISA Kit

MBS705296

48-Strip-Wells

565 USD

ELISA Kit

Human Signal Peptide, CUB and EGF-like Domain-containing Protein 1, SCUBE1 ELISA Kit

signal peptide, CUB domain, EGF-like 1

Human Signal Peptide; CUB and EGF-like Domain-containing Protein 1 (SCUBE1) ELISA Kit; signal peptide-CUB domain-EGF-related 1; signal peptide; CUB domain; EGF-like 1

signal peptide, CUB and EGF-like domain-containing protein 1; Signal peptide, CUB and EGF-like domain-containing protein 1; signal peptide, CUB and EGF-like domain-containing protein 1; signal peptide-CUB domain-EGF-related 1; signal peptide, CUB domain, EGF-like 1

SCUBE1

SCUBE1; SCUBE1

SCUB1_HUMAN

Human

988

This assay has high sensitivity and excellent specificity for detection of human SCUBE1. No significant cross-reactivity or interference between human SCUBE1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.625 ng/ml -40 ng/ml. Sensitivity: 0.156 ng /ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SCUBE1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SCUBE1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SCUBE1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SCUBE1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

120587029

NP_766638.2

NM_173050.3

Q8IWY4

107,910 Da

This gene encodes a cell surface glycoprotein that is a member of the SCUBE (signal peptide, CUB domain, EGF (epidermal growth factor)-like protein) family. Family members have an amino-terminal signal peptide, nine copies of EGF-like repeats and a CUB domain at the carboxyl terminus. This protein is expressed in platelets and endothelial cells and may play an important role in vascular biology. [provided by RefSeq, Oct 2011]

Function: Could function as an adhesive molecule and its matrix bound and soluble fragments may play a critical role in vascular biology.1 PublicationManual assertion based on experiment in:Ref.3

48-Strip-Wells

565 USD

96-Strip-Wells

810

5x96-Strip-Wells

2750

10x96-Strip-Wells

5150